Strain Information | |
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Image | |
BRC No. | RBRC01723 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Dffb<tm1Osa>/OsaRbrc |
Former Common name | CAD KO; CAD Knockout mouse |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Drs. Kohki Kawane and Shigekazu Nagata at Osaka University in 2003. A neomycin cassette replaced exons 1-6 and part of 7 of Dffb gene. The mutant mice were backcrossed to C57BL/6. |
Strain description | B6;129-Dffb<tm1Osa>. CAD, Caspase-activated DNase is an endonuclease that is activated in apoptotic cells. CAD deficient thymocytes did not undergo apoptotic DNA fragmentation, but their DNA was digested by thymic macrophages that effectively phagocytosed the apoptotic thymocytes. This strain is useful for elucidation of the function of CAD gene. |
Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj) Homozygous mutant mice show no gross developmental abnormalities and are fertile with normal fecundity. |
References | Impaired thymic development in mouse embryos deficient in apoptotic DNA degradation. Kawane K, Fukuyama H, Yoshida H, Nagase H, Ohsawa Y, Uchiyama Y, Okada K, Iida T, Nagata S Nat. Immunol., 4, 138-144 (2003). 12524536 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Dffb | DNA fragmentation factor, beta subunit | 4 | Dffb | targeted mutation 1, Shigekazu Nagata | |||
neo | neomycin resistance gene (E. coli) | 4 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Dffb (CAD) genomic DNA |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. immunol., 4, 138-144 (2003). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Shigekazu Nagata (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Gao L, Huang K, Jiang DS, Liu X, Huang D, Li H, Zhang XD, Huang K. Novel role for caspase-activated DNase in the regulation of pathological cardiac hypertrophy. Hypertension 65(4) 871-81(2015) 25646292 |