Strain Data Sheet



BRC No.RBRC01709
SpeciesMus musculus
Strain nameB6.Cg-Tg(Mt1-RET)304Ina/B6
Former Common name304-B6 RET
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack with black skin (hyperpigmented skin) [a/a B/B C/C Tg/+]
black [a/a B/B C/C +/+]
Strain developmentDeveloped by Masashi Kato, Nagoya University Graduate School of Medicine. The Mt1-RET transgene was injected into the fertilized eggs of (BALB/c x C57BL/6) x BALB/c mice. C57BL/6 and BALB/c mixed background.
Strain descriptionMt1-Ret 304 transgenic mice. The mice express the human RET under the control of the mouse MT1 promoter/enhancer. This Mt1-RET 304 strain is a model of cutaneous melanosis, and develops benign tumors 100% on the skin. Of these tumors, 65% transform to malignant tumors in ten to twelve months after the birth. Tg homozygous mice are embryonic lethal.
Colony maintenanceCarrier x Noncarrier
ReferencesEMBO J., 10, 3167-3175 (1991). 1915289

Health Report

Examination Date / Room / Rack2015/11/09Room:2-3Rack:E


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tg(Mt1-RET)304Inatransgene insertion 304, Izumi NakashimaUNTg(Mt1-RET)304Ina

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
RETrecombinant RET proto-oncogene (human)UNRETRET51, RET9mouse metallothionein 1 promoter/enhancer

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 splicing signal-polyadenylation siteUN


Donor DNAhuman RFP/RET cDNA, mouse MT-I promoter-enhancer, SV40 splicing signal-polyadenylation site, vector sequence
Research applicationCancer Research
Dermatology Research
Mouse Models for Human Disease
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorMasashi Kato (Chubu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data