Strain Data Sheet

RBRC01680

Strain Information

Image
BRC No.RBRC01680
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>/JtakRbrc
Former Common nameGene trap of Smoc1 (SPARC related modular calcium binding 1), PV384
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain development開発年：2005年開発者：竹田潤二先生機関名：大阪大学先端科学イノベーションセンター　バイオ・生命系分野Mixed background
Strain descriptionGene trap 型の Sleeping Beauty transposon vector の germline での転移によって遺伝子変異を導入。Transposon vector については、参考文献を参照。
Colony maintenance繁殖効率：A
ReferencesNat. Methods, 2, 763-769 (2005). 16179923
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Smoc1SPARC related modular calcium binding 112Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>gene trap lacZ/GFP PV384, Junji Takeda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BCL2Splice acceptor (human bcl-2)12BCL2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)12CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (human)12GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA12HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)12internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pgk1mouse pgk promoter12Pgk1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor (Salmon)12SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Splice donorSleeping Beauty transposase12Splice donor

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Splice donorSplice donor (mouse hprt)12Splice donor

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)12

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)12neo; neomycin;

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mouse synaptonemal protein 1 promoter

Ordering Information

供与核酸Salmon Sleeping Beauty transposon, Salmon Sleeping Beauty transposase, Jellyfish GFP gene, E. coli LacZ gene, E. coli neo gene, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Human bcl-2 splice acceptor, Mouse hprt splice donor, Cytomegalovirus enhancer, Chicken beta-actin promoter, Mouse pgk promoter, Rabbit beta-globin polyadenylation signal sequence, Mouse synaptonemal protein 1 promoter, Human growth hormone polyadenylation signal sequence, pBluescript
Research applicationFluorescent Proteins/lacZ System
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Methods., 2, 763-769 (2005).
非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。
Depositor竹田　潤二（国立大学法人大阪大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Takeda Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Okada I, Hamanoue H, Terada K, Tohma T, Megarbane A, Chouery E, Abou-Ghoch J, Jalkh N, Cogulu O, Ozkinay F, Horie K, Takeda J, Furuichi T, Ikegawa S, Nishiyama K, Miyatake S, Nishimura A, Mizuguchi T, Niikawa N, Hirahara F, Kaname T, Yoshiura K, Tsurusaki Y, Doi H, Miyake N, Furukawa T, Matsumoto N, Saitsu H.
SMOC1 is essential for ocular and limb development in humans and mice.
Am J Hum Genet 88(1) 30-41(2011) 21194678

Delgado Lagos F, Elgheznawy A, Kyselova A, Meyer Zu Heringdorf D, Ratiu C, Randriamboavonjy V, Mann AW, Fisslthaler B, Siragusa M, Fleming I.
Secreted modular calcium-binding protein 1 binds and activates thrombin to account for platelet hyperreactivity in diabetes.
Blood 137(12) 1641-1651(2021) 33529332

Awwad K, Hu J, Shi L, Mangels N, Abdel Malik R, Zippel N, Fisslthaler B, Eble JA, Pfeilschifter J, Popp R, Fleming I.
Role of secreted modular calcium-binding protein 1 (SMOC1) in transforming growth factor β signalling and angiogenesis.
Cardiovasc Res 106(2) 284-94(2015) 25750188