Strain Data Sheet

RBRC01578

Strain Information

Image
BRC No.RBRC01578
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pik3r1<tm1Tka>/Rbrc
Former Common nameC57BL/6-PI3K KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain development1999年に東大の門脇 孝先生と共同で作成し、その後 C57BL/6NTac と BALB/cATac に12代戻し交配したもの
Strain descriptionPI3K, phosphoinositide-3-kinase gene is a lipid kinases that catalyze the addition of a phosphate molecule specifically to the 3-position of the inositol ring of phosphoinositides. This strain is useful for elucidating of the rule of PI3K in various phases of immune responses. Homozygous mutant females were sterile.
Colony maintenanceSince most of homozygous females are sterile, we maintain the colony by mating heterozygous females with homozygous males.
ReferencesScience, 283, 390-392 (1999). 9888854
Nat. Genet., 21, 230-235 (1999). 9988280

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pik3r1phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)13Pik3r1<tm1Tka>targeted mutation 1, Takashi Kadowakip50alpha, p55alpha, p85alpha, PI3K

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13neo; neomycin;herpes simplex virus thymidine kinase (HSV TK) poly A

Ordering Information

供与核酸E. coli neo, HSV TK polyA, mouse PI3K genomic DNA
Research application
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Science, 283, 390-392 (1999). Nat. genet., 21, 230-235 (1999).
研究成果の公表にあたって謝辞の表明を必要とする。
Depositor小安　重夫（慶應義塾大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data