Strain Data Sheet

RBRC01577

Strain Information

Image
BRC No.RBRC01577
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Pik3r1<tm1Tka>/Rbrc
Former Common nameBALB/c-PI3K KO
H-2 Haplotype
ES Cell line
Background strainBALB/cAnNCrlCrlj
Appearancealbino [A/A b/b c/c]
Strain development1999年に東大の門脇 孝先生と共同で作成し、その後 C57BL/6NTac と BALB/cATac に12代戻し交配したもの
Strain descriptionC.Cg-Pik3r1<tm1Tka>. The PI3K, phosphoinositide-3-kinase gene is a key enzyme producing phospholipid second messengers and plays an important role in various signal transduction pathways. This knockout mouse strain is useful for investigating the function of the PI3K gene.
Colony maintenanceSince most of homozygous females are sterile, we maintain the colony by mating heterozygous females with homozygous males.
ReferencesNat. Genet., 21, 230-235 (1999). 9988280
Science, 283, 390-392 (1999). 9888854

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pik3r1phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)13Pik3r1<tm1Tka>targeted mutation 1, Takashi Kadowakip50alpha, p55alpha, p85alpha, PI3K

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13neo; neomycin;

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly AHSV TK polyA13poly A

Ordering Information

供与核酸mouse PI3K genomic DNA, E. coli neo, HSV TK polyA
Research application
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Science, 283, 390-392 (1999). Nat. genet., 21, 230-235 (1999).
研究成果の公表にあたって謝辞の表明を必要とする。
Depositor小安　重夫（慶應義塾大学）
Strain Status凍結胚のアイコン凍結胚
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Umehara D, Watanabe S, Ochi H, Anai Y, Ahmed N, Kannagi M, Hanson C, Ruscetti S, Nishigaki K.
Role of phosphatidylinositol 3-kinase in friend spleen focus-forming virus-induced erythroid disease.
J Virol 84(15) 7675-82(2010) 20504929