Strain Data Sheet

RBRC01558

Strain Information

Image
BRC No.RBRC01558
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nrxn3<Tn(sb-lacZ,GFP)OR5Jtak> Nrxn1<Tn(sb-lacZ,GFP)OR85Jtak>/JtakRbrc
Former Common nameOR5/85
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development開発年：2005年開発者：竹田潤二先生機関名：大阪大学先端科学イノベーションセンター　 バイオ・生命系分野Mixed background
Strain descriptionGene trap 型の Sleeping Beauty transposon vector のgermline での転移によって遺伝子変異を導入。Vector の挿入部位の情報は、www25.casi.osaka-u.ac.jp のトランスポゾンデータベースを参照。OR5 (Nrxn3) と OR85 (Nrxn1) の2つの系統を交配後、両方の変異遺伝子について heterozygous となったマウスを寄託。
Colony maintenance繁殖効率：ANcurcxin 1, 3の両遺伝子についてのホモは離乳前に死亡。
ReferencesNat. Methods, 2, 763-769 (2005). 16179923
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nrxn3neurexin III12Nrxn3<Tn(sb-lacZ,GFP)OR5Jtak>transposon insertion OR5, Junji Takeda4933401A11Rik, 9330112C09Rik, D12Bwg0831e, neurexin III alpha, neurexin III alpha, neurexin III beta, neurexin III beta

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BCL2Splice acceptor (human bcl-2)12BCL2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)12CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)12internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Splice donorSplice donor (mouse hprt)12Splice donor

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)12

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nrxn1neurexin I17Nrxn1<Tn(sb-lacZ,GFP)OR85Jtak>transposon insertion OR85, Junji Takeda1700062G21Rik, 9330127H16Rik, A230068P09Rik, alpha-latrotoxin receptor (calcium-dependent), mKIAA0578, neurexin I alpha, neurexin I alpha, neurexin I alpha, neurexin I beta, neurexin I beta, neurexin I beta

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BCL2Splice acceptor (human bcl-2)17BCL2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)17CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)17internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Splice donorSplice donor (mouse hprt)17Splice donor

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)17

Ordering Information

供与核酸pTrans-SA, Encephalomyocarditis virus (EMCV) internal ribosomal entrysite (ires), E. coli lacZ, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish EGFP cDNA, SD, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationFluorescent Proteins/lacZ System
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Methods., 2, 763-769 (2005).
非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。
Depositor竹田　潤二（国立大学法人大阪大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Takeda Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data