Strain Data Sheet

RBRC01556

Strain Information

Image
BRC No.RBRC01556
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nell2<Tn(sb-lacZ,GFP)OR238Jtak>/JtakRbrc
Former Common nameGene trap of Nell2 (nel-like 2 homolog), OR238
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development開発年：2005年開発者：竹田潤二先生機関名：大阪大学先端科学イノベーションセンター　バイオ・生命系分野Mixed background
Strain descriptionGene trap 型の Sleeping Beauty tranposon vector の germline での転移によって遺伝子変異を導入。Transposon vector については、参考文献を参照。Vector 挿入部位の情報は、www25.casi.osaka-u.ac.jp のトランスポゾンデータベースを参照。
Colony maintenance繁殖効率：A
ReferencesMol. Cell. Biol., 23, 9189-9207 (2003). 14645530
Nat. Methods, 2, 763-769 (2005). 16179923

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nell2nel-like 2 homolog15Nell2<Tn(sb-lacZ,GFP)OR238Jtak>transposon insertion OR238, Junji Takeda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BCL2Splice acceptor (human bcl-2)15BCL2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)15CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (human)15GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA15HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)15internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SBSleeping Beauty transposon15SB

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Splice donorSplice donor (mouse hprt)15Splice donor

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)15

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15neo; neomycin;

Ordering Information

供与核酸Salmon Sleeping Beauty transposon*, Salmon Sleeping Beauty transposase*, Human bcl-2 splice acceptor, Encephalomyocarditis virus (EMCV)internal ribosomal entry site (ires), E. coli LacZ gene, Rabbit beta-globin polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish GFP gene, Mouse hprt splice donor, mouse Pgk promoter*, Mouse synaptonemal protein 1 promoter*, Human growth hormone polyadenylation signal*, pBluescript*[E. coli neomycin resistance gene was not detected by PCR. Detection of introduced genes (asterisk) were not tested.]
Research applicationFluorescent Proteins/lacZ System
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Methods., 2, 763-769 (2005).
非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。
Depositor竹田　潤二（国立大学法人大阪大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Takeda Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data