Strain Data Sheet

RBRC01556

Strain Information

Image
BRC No.RBRC01556
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nell2<Tn(sb-lacZ,GFP)OR238Jtak>/JtakRbrc
Former Common nameGene trap of Nell2 (nel-like 2 homolog), OR238
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesNat. Methods, 2, 763-769 (2005). 16179923
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nell2nel-like 2 homolog15Nell2<Tn(sb-lacZ,GFP)OR238Jtak>transposon insertion OR238, Junji Takeda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BCL2Splice acceptor (human bcl-2)15BCL2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)15CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (human)15GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA15HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)15internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SBSleeping Beauty transposon15SB

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Splice donorSplice donor (mouse hprt)15Splice donor

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)15

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15neo; neomycin;

Ordering Information

Donor DNASalmon Sleeping Beauty transposon*, Salmon Sleeping Beauty transposase*, Human bcl-2 splice acceptor, Encephalomyocarditis virus (EMCV)internal ribosomal entry site (ires), E. coli LacZ gene, Rabbit beta-globin polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish GFP gene, Mouse hprt splice donor, mouse Pgk promoter*, Mouse synaptonemal protein 1 promoter*, Human growth hormone polyadenylation signal*, pBluescript*[E. coli neomycin resistance gene was not detected by PCR. Detection of introduced genes (asterisk) were not tested.]
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorJunji Takeda (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Takeda Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data