Strain Data Sheet

RBRC01550

Strain Information

Image
BRC No.RBRC01550
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Syt5<Tn(sb-lacZ,GFP)OR153Jtak>/JtakRbrc
Former Common nameGene trap of Syt5 (synaptotagmin V), OR153
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesMol. Cell. Biol., 23, 9189-9207 (2003). 14645530
Nat. Methods, 2, 763-769 (2005). 16179923

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Syt5synaptotagmin V7Syt5<Tn(sb-lacZ,GFP)OR153Jtak>transposon insertion OR153, Junji Takeda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BCL2Splice acceptor (human bcl-2)7BCL2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)7CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (human)7GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA7HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)7internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SBSleeping Beauty transposase7SB

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SBSleeping Beauty transposon7SB

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Splice donorSplice donor (mouse hprt)7Splice donor

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mouse synaptonemal protein 1 promoter7

Ordering Information

Donor DNASleeping Beauty トランスポゾン, Sleeping Beauty トランスポゼース, GFP 遺伝子, LacZ 遺伝子, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires),Human bcl-2 スプライスアクセプター, Mouse hprt スプライスドナー, Cytomegalovirus エンハンサー, Chicken beta-actin プロモーター, Rabbit beta-globin ポリA付加シグナル, Mouse synaptonemal protein 1 プロモーター, Human growth hormone ポリ A付加シグナル, pBluescript
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorJunji Takeda (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Takeda Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data