Strain Data Sheet

RBRC01544

update : 2024-04-19

Strain Information
Image
BRC No.	RBRC01544
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6.129P2-Sirpa<tm1Nog>/Rbrc
Former Common name	SHPS-1 Knockout mice
H-2 Haplotype
ES Cell line	E14.1 [129P2/OlaHsd]
Background strain	C57BL/6NJcl
Appearance	black
Strain development	神戸大学で作製。10世代　C57BL/6Nにバッククロス。
Strain description	129/Sv 系統由来 ES 細胞 (E14.1) にターゲティングベクターを導入し作製。膜タンパク質SHPS-1 の機能解析に使用。
Colony maintenance	繁殖効率：B野生型に比べ、繁殖効率が劣る印象、定量的データはなし。繁殖成績が低い
References	EMBO J., 19, 6721-6731 (2000). 11118207 J. Biol. Chem., 277 (42) 39833-9 (2002). 12167615

Health Report
Examination Date / Room / Rack

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Sirpasignal-regulatory protein alpha2Sirpa<tm1Nog>targeted mutation 1, Tetsuya NoguchiBit, CD172a, P84, SHPS-1, SIRP; Ptpns1;protein tyrosine phosphatase, non-receptor type substrate 1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)2neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene

Gene info

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Sirpasignal-regulatory protein alpha2Sirpa<tm1Nog>targeted mutation 1, Tetsuya NoguchiBit, CD172a, P84, SHPS-1, SIRP; Ptpns1;protein tyrosine phosphatase, non-receptor type substrate 1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

neoneomycin resistance gene (E. coli)2neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information
供与核酸	mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Sirpa genomic DNA
Research application
提供条件	条件を付加する。利用者は事前に寄託者の提供承諾書を得る。群馬大学生体調節研究所に受領書を提出する。
Depositor	的崎　尚（国立大学法人群馬大学）
Strain Status	凍結胚凍結精子
Strain Availability	凍結胚より作出したマウスを2~4ヶ月以内に提供可能凍結精子を1ヶ月以内に提供可能凍結胚を1ヶ月以内に提供可能
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Lab HP Genotyping protocol -PCR- Genetic Background

BRC mice in Publications

Mark Kearns, William J. Janssen, and Peter M. Henson Signal Inhibitory Regulatory Protein Alpha Influences Macrophage Clearance From The Alveolar Space After An Inflammatory Stimulus. Am. J. Respir. Crit. Care Med. 181 A2761(2010) Cham LB, Torrez Dulgeroff LB, Tal MC, Adomati T, Li F, Bhat H, Huang A, Lang PA, Moreno ME, Rivera JM, Galkina SA, Kosikova G, Stoddart CA, McCune JM, Myers LM, Weissman IL, Lang KS, Hasenkrug KJ. Immunotherapeutic Blockade of CD47 Inhibitory Signaling Enhances Innate and Adaptive Immune Responses to Viral Infection. Cell Rep 31(2) 107494(2020) 32294445 Li LX, Atif SM, Schmiel SE, Lee SJ, McSorley SJ. Increased susceptibility to Salmonella infection in signal regulatory protein α-deficient mice. J Immunol 189(5) 2537-44(2012) 22851710 Wang L, Lu Y, Deng S, Zhang Y, Yang L, Guan Y, Matozaki T, Ohnishi H, Jiang H, Li H. SHPS-1 deficiency induces robust neuroprotection against experimental stroke by attenuating oxidative stress. J Neurochem 122(4) 834-43(2012) 22671569 Sarute N, Cheng H, Yan Z, Salas-Briceno K, Richner J, Rong L, Ross SR. Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways. PLoS Pathog 17(6) e1009662(2021) 34097709 Christina Wenzek, Philine Steinbach, Florian Wirsdörfer, Kathrin Sutter, Julia D. Boehme, Robert Geffers, Robert Klopfleisch, Dunja Bruder, Verena Jendrossek, Jan Buer, Astrid M. Westendorf, Torben Knuschke CD47 restricts antiviral function of alveolar macrophages during influenza virus infection iScience 25 105540(2022) 36437873

BRC mice in Publications

Mark Kearns, William J. Janssen, and Peter M. Henson
Signal Inhibitory Regulatory Protein Alpha Influences Macrophage Clearance From The Alveolar Space After An Inflammatory Stimulus.
Am. J. Respir. Crit. Care Med. 181 A2761(2010)

Cham LB, Torrez Dulgeroff LB, Tal MC, Adomati T, Li F, Bhat H, Huang A, Lang PA, Moreno ME, Rivera JM, Galkina SA, Kosikova G, Stoddart CA, McCune JM, Myers LM, Weissman IL, Lang KS, Hasenkrug KJ.
Immunotherapeutic Blockade of CD47 Inhibitory Signaling Enhances Innate and Adaptive Immune Responses to Viral Infection.
Cell Rep 31(2) 107494(2020) 32294445

Li LX, Atif SM, Schmiel SE, Lee SJ, McSorley SJ.
Increased susceptibility to Salmonella infection in signal regulatory protein α-deficient mice.
J Immunol 189(5) 2537-44(2012) 22851710

Wang L, Lu Y, Deng S, Zhang Y, Yang L, Guan Y, Matozaki T, Ohnishi H, Jiang H, Li H.
SHPS-1 deficiency induces robust neuroprotection against experimental stroke by attenuating oxidative stress.
J Neurochem 122(4) 834-43(2012) 22671569

Sarute N, Cheng H, Yan Z, Salas-Briceno K, Richner J, Rong L, Ross SR.
Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways.
PLoS Pathog 17(6) e1009662(2021) 34097709

Christina Wenzek, Philine Steinbach, Florian Wirsdörfer, Kathrin Sutter, Julia D. Boehme, Robert Geffers, Robert Klopfleisch, Dunja Bruder, Verena Jendrossek, Jan Buer, Astrid M. Westendorf, Torben Knuschke
CD47 restricts antiviral function of alveolar macrophages during influenza virus infection
iScience 25 105540(2022) 36437873

Strain Information

Health Report

Gene

Ordering Information

BRC mice in Publications