Strain Data Sheet

RBRC01508

Information1

Image
BRC No.RBRC01508
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Camk2a-cre,-tTA)1Tyag/TyagRbrc
Former Common nameCaM-Cre 1 mice
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Makoto Sanbo and Takeshi Yagi, National Institute for Physiological Sciences, Japan.
Strain descriptionThis transgenic mouse expresses the cre-recombinase under the control of the calcium/calmodulin-dependent protein kinase II alpha promoter. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineeered mice that have floxed genes.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6NCrlCrlj)
References

Health Report

Examination Date / Room / Rack2007/07/17Room:4-ARack:H
2007/05/22Room:4-ARack:H
2007/05/21Room:4-ARack:H
2007/03/19Room:4-ARack:H
2007/01/15Room:4-ARack:H
2006/11/13Room:4-ARack:H
2006/09/19Room:4-ARack:H
2006/09/19Room:4-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (human)UNGH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UNinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcremouse Camk2a promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain

Information2

Donor DNAmouse CaM Kinase2 promoter, P1 phage hnCre recombinase, E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), human Growth Hormone-poly A
Research applicationCell Biology Research
Cre/loxP system
Tet system
Specific Term and Conditions1. The RECIPIENT should negotiate with Dr. Takeshi Yagi and Makoto Sanbo in the case of application for any patents with the results from the use of the BIOLOGICAL RESOURCE.
2. The RECIPIENT should contact with Dr. Takeshi Yagi and Makoto Sanbo in regard to co-authorship.
DepositorKazuhiro Ikenaka (National Institute for Physiological Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

No Data