Strain Data Sheet

RBRC01498

Strain Information

Image
BRC No.RBRC01498
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Camk2-mutPolg)1Bsi
Former Common nameBipolar Disorder Model Mouse
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Drs. Takaoki Kasahara and Tadafumi Kato at RIKEN BSI in 2001. The mutant POLG coding fragment including the CaMKII alpha promoter was injected into the pronuclei of C57BL/6J fertilized eggs.
Strain descriptionBipolar disorder, a mood disorder marked by alternating periods of manic and depressive episodes, affects approximately 1% of the global population. Mitochondrial dysfunction has been seen in patients with bipolar disorder. Moreover, patients with mitochondrial disorders sometimes display mood disorders. This transgenic mice express mouse mitochondrial DNA polymerase (POLG) having a point mutation D181A under the control of CaMKII alpha promoter. The mutant mice exhibited characteristic behavioral phenotypes in accordance with criteria for the validation as an animal model of mood disorder. This strain is useful to investigate the cause of bipolar disorder and to develop new mood stabilizers to treat bipolar disorder.
Colony maintenanceBackcross to C57BL/6 (hemizygote male x 57BL/6JJcl female)Don't use Tg female for breeding in order to prevent transmission of mutated mtDNA.
References
Mice with neuron-specific accumulation of mitochondrial DNA mutations show mood disorder-like phenotypes.
Kasahara T, Kubota M, Miyauchi T, Noda Y, Mouri A, Nabeshima T, Kato T
Mol. Psychiatry, 11, 577-593 (2006). 16619054

Health Report

Examination Date / Room / Rack2024/10/21Room:3-CRack:F

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Polgpolymerase (DNA directed), gammaUNPolgmouse Camk2a promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal circadian behavior(MP:0020467)

  • abnormal emotion/affect behavior(MP:0002572)

  • abnormal mitochondrial chromosome morphology(MP:0011638)

  • decreased body weight(MP:0001262)

  • decreased food intake(MP:0011940)
    • more 3 phenotypes
  • decreased serotonin level(MP:0010070)

  • impaired exercise endurance(MP:0012106)

  • increased startle reflex(MP:0001488)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse CaMKIIα promoter , mouse Polg (mitochondrial DNA polymerase γ) mutant cDNA, SV40 polyA
    Research applicationCell Biology Research
    Mouse Models for Human Disease
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Molecular Psychiatry, 11, 577-593 (2006). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
    The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE when the RECIPIENT ordered the BIOLOGICAL RESOURCE before the publication of the paper indicated above.
    DepositorTadafumi Kato (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month May 2006

    BRC mice in Publications

    No Data