Strain Information | |
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Image | |
BRC No. | RBRC01491 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Lrrn4<tm1Ami>/Rbrc |
Former Common name | NLRR4-KO(B6); B6.Cg-B430119L13Rik<tm>, B6;B6CB-Lrrn4<tm1>/Rbrc |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Drs. Takayoshi Bando and Atsushi Miyajima at the University of Tokyo in 2001. The TT2 ES cells originated from an F1 embryo between C57BL/6 and CBA mice were used. The targeting construct contains relpaced exons with the beta-galactosidase and the neomycin resistance genes by homologous reconbination. The NLRR4 deficient mice were backcrossed to C57BL/6JJcl. |
Strain description | B6.Cg-B430119L13Rik<tm>. NLRR4 is a member of the neuronal leucine-rich repeat protein family. NLRR proteins are type I transmembrane proteins with leucine-rich repeats and a fibronectine type III repeat, and expressed in neuronal tissues. NLRR4 deficient mice have a severe damage to their long-term memory in hippocampus-dependent tasks, while their hippocampal long-term potentiation was intact. NLRR4 plays a role in hippocamus-dependent long-lasting memory. |
Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6J)Homozygous deficient mice are viable and fertile. |
References | Mol. Cell. Biol., 25, 4166-4175 (2005). 15870286 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)2neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, E. coli lacZ, poly A, mouse Lrrn4 genomic DNA |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 10, 4160-4175 (2005).The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. |
Depositor | Atsushi Miyajima (The University of Tokyo) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |