Strain Information | |
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Image | |
BRC No. | RBRC01474 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Fas<tm1Osa>/OsaRbrc |
Former Common name | B6 Fas-KO |
H-2 Haplotype | |
ES Cell line | E14.1 [129P2/OlaHsd] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Drs. Masashi Adachi and Shigekazu Nagata at Osaka University Medical School in 1995. |
Strain description | Fas is a cell surface receptor belonging to the tumor necrosis factor receptor superfamily which transduces apoptotic signals in many cell types. Fas deficient mutant mice show lymphoma and autoimmune disorders. In a few month after birth, the mice develop lymphadenoma and splenomegaly by an accumulation of abnormal polyclonal T cells with the CD4-CD8- double negative. |
Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)Homozygous mutant mice show lymphoma and autoimmune disorders. 50% dead at about 5 months of age. |
References | Targeted mutation in the Fas gene causes hyperplasia in peripheral lymphoid organs and liver. Adachi M, Suematsu S, Kondo T, Ogasawara J, Tanaka T, Yoshida N, Nagata S Nat. Genet., 11(3), 294-300 (1995). 7581453 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Fas | Fas cell surface death receptor | 19 | Fas | targeted mutation 1, Osaka University Medical School | |||
lacZ | beta-galactosidase (E. coli) | 19 | |||||
neo | neomycin resistance gene (E. coli) | 19 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 16 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Fas genomic DNA |
Research application | Apoptosis Research Immunology and Inflammation Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Genet., 11(3), 294-300 (1995). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Shigekazu Nagata (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Jeong D, Kim HS, Kim HY, Kang MJ, Jung H, Oh Y, Kim D, Koh J, Cho SY, Jeon YK, Lee EB, Lee SH, Shin EC, Kim HM, Yi EC, Chung DH. Soluble Fas ligand drives autoantibody-induced arthritis by binding to DR5/TRAIL-R2. Elife 10 (2021) 34223817 |