Strain Data Sheet

RBRC01465

Strain Information

Image
BRC No.RBRC01465
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6(Cg)-Cacna1g<tm1.3Ksak>/KsakRbrc
Former Common nameCa1G KO, C57BL/6-Cacna1g<-/+>
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain development開発者：（ターゲティングベクター）金沢大学大学院医学系研究科　新石健二先生， 狩野方伸先生（マウス系統）新潟大学脳研究所細胞神経生物　阿部学先生， 夏目里恵先生， 辻田実加先生， 崎村建司先生開発年：2002年　キメラマウス取得。Bruce株（gifted by Dr. Colin L. Stewart）を用いて、相同組み換え体を取得．キメラマウスを作製。C57BL/6との F1からTLCN-Creマウス系統(文献参照)とのF2を得た．F2を相互に交配し，F3でCre遺伝子を持たないhetero KO個体を得た．このF3の相互交配によりF4でNull KO個体を得た．Null KOの相互交配により系統維持．現在はF6．
Strain descriptionCacna1gのexon5の両側にloxP配列を挿入した。TLCN-Creマウス系統との交配によりexon5欠失個体を得た． Cacna1g null KOマウスとして解析に用いることができる．
Colony maintenance繁殖効率：A
ReferencesEur. J. Neurosci., 13, 179-189 (2001). 11135016
Biochem. Biophys. Res. Commun., 318, 665-672 (2004). 15144889

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cacna1gcalcium channel, voltage-dependent, T type, alpha 1G subunit11Cacna1g<tm1.3Ksak>targeted mutation 1.3, Kenji Skimura[a]1G, a1G, alpha-1G, Cav3.1d, mKIAA1123

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Ordering Information

供与核酸Phage P1 loxP site, mouse Cacna1g genomic DNA
Research applicationCre/loxP system
提供条件条件を付加する。
・The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
・The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC.
・Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
Depositor崎村　建司（国立大学法人新潟大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Matsumoto-Makidono Y, Nakayama H, Yamasaki M, Miyazaki T, Kobayashi K, Watanabe M, Kano M, Sakimura K, Hashimoto K.
Ionic Basis for Membrane Potential Resonance in Neurons of the Inferior Olive.
Cell Rep 16(4) 994-1004(2016) 27425615

Tracy ME, Tesic V, Stamenic TT, Joksimovic SM, Busquet N, Jevtovic-Todorovic V, Todorovic SM.
CaV3.1 isoform of T-type calcium channels supports excitability of rat and mouse ventral tegmental area neurons.
Neuropharmacology 135 343-354(2018) 29578032

Handforth A, Homanics GE, Covey DF, Krishnan K, Lee JY, Sakimura K, Martin FC, Quesada A.
T-type calcium channel antagonists suppress tremor in two mouse models of essential tremor.
Neuropharmacology 59(6) 380-7(2010) 20547167

Choi S, Yu E, Hwang E, Llinás RR.
Pathophysiological implication of CaV3.1 T-type Ca2+ channels in trigeminal neuropathic pain.
Proc Natl Acad Sci U S A 113(8) 2270-5(2016) 26858455

Choi S, Yu E, Lee S, Llinás RR.
Altered thalamocortical rhythmicity and connectivity in mice lacking CaV3.1 T-type Ca2+ channels in unconsciousness.
Proc Natl Acad Sci U S A 112(25) 7839-44(2015) 26056284