Strain Data Sheet

RBRC01464

Strain Information

Image
BRC No.RBRC01464
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6(Cg)-Cacna1g<tm1.1Ksak>/KsakRbrc
Former Common nameCa1G(flox), C57BL/6-Cacna1g<flox/+>
H-2 Haplotype
ES Cell lineBruce (gifted by Dr. Colin L. Stewart)
Background strain
Appearance
Strain development開発者：（ターゲティングベクター）金沢大学大学院医学系研究科　新石健二先生， 狩野方伸先生（マウス系統）新潟大学脳研究所細胞神経生物　阿部学先生， 夏目里恵先生， 辻田実加先生， 崎村建司先生開発年：2002年　キメラマウス取得。Bruce株（gifted by Dr. Colin L. Stewart）を用いて、相同組み換え体を取得．C57BL/6とのF1をFLPマウス系統(文献参照)と交配し，このF2をC57BL/6と交配してNeoカセットを除去したF3を得た．F3の相互交配により繁殖．現在の世代数はF6．
Strain descriptionCacna1gのexon5の両側にloxP配列を挿入した。FLPリコンビネース発現マウスとの交配によりpgk-neoカセットを除去した。exon5と3’側のloxPの間にfrt配列をもつ。Creリコンビナーゼ発現マウスとの交配などにより、コンディショナルノックアウトマウスとして用いることができる
Colony maintenance
References
Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting.
Takeuchi T, Nomura T, Tsujita M, Suzuki M, Fuse T, Mori H, Mishina M
Biochem. Biophys. Res. Commun., 293, 953-957 (2002). 12051751

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cacna1g
MGI:1201678		calcium channel, voltage-dependent, T type, alpha 1G subunit11Cacna1g<tm1.1Ksak> targeted mutation 1.1, Kenji Skimura
  • spinocerebellar ataxia 42, early-onset, severe, with neurodevelopmental deficits(MedGEN)

  • spinocerebellar ataxia type 42(MedGEN)
    • Frt yeast FRT (flippase recombination target) site11Frt
    loxP phage P1 loxP11loxP
    loxP phage P1 loxP11loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal single cell response(MP:0003463)

  • impaired behavioral response to xenobiotic(MP:0009747)
    • Detailed phenotype data

    Ordering Information

    供与核酸Phage P1 loxP sites, S. cerevisiae 2μ plasmid FRT (flipase recombination target) site, mouse Cacna1g genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    提供条件・The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
    ・The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC.
    ・Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
    Depositor崎村　建司（国立大学法人新潟大学）
    Strain Status凍結胚のアイコン凍結胚
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data