Strain Information | |
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Image | |
BRC No. | RBRC01464 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6(Cg)-Cacna1g<tm1.1Ksak>/KsakRbrc |
Former Common name | Ca1G(flox), C57BL/6-Cacna1g<flox/+> |
H-2 Haplotype | |
ES Cell line | Bruce (gifted by Dr. Colin L. Stewart) |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Kenji Sakimura, Brain Research Institute, Nigata University in 2002. The targeted allele of this strain was flanked exon 5 with loxP sites and a FRT flanked Neo resistant gene driven by the PGK promotor. The FRT-flanked neo was excised by crossing with mice expressing Flp. |
Strain description | Cacna1g gene is a member of the low-voltage-activated calcium channel that plays a critcal role in calcium ion transport in nerve, muscle and endocrine cells. The targeted allele of this strain was flanked exon 5 with loxP sites. The FRT-flanked neo was excised by crossing with mice expressing Flp. Conditional Cacna1g deficient mice can be generated by crossing with tissue-specific Cre mice. |
Colony maintenance | |
References | Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting. Takeuchi T, Nomura T, Tsujita M, Suzuki M, Fuse T, Mori H, Mishina M Biochem. Biophys. Res. Commun., 293, 953-957 (2002). 12051751 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Cacna1g | calcium channel, voltage-dependent, T type, alpha 1G subunit | 11 | Cacna1g | targeted mutation 1.1, Kenji Skimura | |||
Frt | yeast FRT (flippase recombination target) site | 11 | Frt | ||||
loxP | phage P1 loxP | 11 | loxP | ||||
loxP | phage P1 loxP | 11 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, S. cerevisiae 2μ plasmid FRT (flipase recombination target) site, mouse Cacna1g genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | ・The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. ・The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC. ・Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice. |
Depositor | Kenji Sakimura (Niigata University) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |