Strain Data Sheet

RBRC01400

Strain Information

Image
BRC No.RBRC01400
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(CAG-Ptafr)1Tksh/TkshRbrc
Former Common namePAFR-Tg, Platelet-activating factor (PAF) receptor transgenic mouse
H-2 Haplotype
ES Cell line
Background strainCrlj:B6D2F1
Appearance
Strain developmentDeveloped by Dr. Satoshi Ishii at the University of Tokyo in 1997. A transgenic construct containing a guinea-pig PAFR gene under the control of the a chiken beta actin promotor and the CMV immediate early enhancer, was introduced into the pronuclei of fertilized eggs of B6D2F2. The transgenic mice were mated with B6D2F1.
Strain descriptionPlatelet-activating factor (PAF) is a phospholipid mediator with pleiotropic and potent biological effects on a variety of cells and tissues. This transgenic mice showed a bronchial hyperreactivity, increased endotoxin lethality and melanocytic tumorigenesis. This strain is useful to elucidating the pathophysiological roles of PAF and PAFR in vivo.
Colony maintenance繁殖成績が低い雄のヘテロマウスを雌のBDF1と交配して、ヘテロマウスで継代する。1/3から1/4の確率でTgマウスが生まれる。雌のHeteroマウスからはほとんどTgマウスが生まれないので、ホモマウスを得ることは非常に難しいと思われる。かつて行ったC57BL/6マウスとの戻し交配では、N8で雄の妊孕性が低下しラインが絶えてしまった。
ReferencesEMBO J., 16, 133-142 (1997). 9009274

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ptafrplatelet-activating factor receptor (guinea-pig)UNPtafrPAF receptor, PAFRCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Ordering Information

供与核酸CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), guinea-pig Platelet-activating factor (PAF) receptor cDNA
Research application
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。EMBO J., 16, 133-142 (1997).
Depositor清水　孝雄（国立大学法人東京大学）
Strain Status凍結胚のアイコン凍結胚
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data