Strain Data Sheet

RBRC01388

Strain Information

Image
BRC No.RBRC01388
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Keap1<tm1Mym>/MymRbrc
Former Common nameKeap1 knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain development筑波大学　TARA センター　山本プロジェクト
Strain descriptionKeap1 gene is a cytoplasmic protein, acts as a negative regulator of Nrf2 and as a sensor of xenobiotic and oxidative stresses. Double knockout mice of Nrf2 and Keap1 demonstrated that Nrf2 is a primary target of Keap1. Nrf2-Keap1 system is one of the major cellular protection mechanisms against xenobiotic and oxidative stresses. Keap1 homozygous mutant mice exhibit scaly skin, hyperkeratosis in the esophague and forestomatch, and died postnatally.
Colony maintenanceホモマウスは致死なので、遺伝子型のヘテロのマウスを維持。
ReferencesNat. Genet., 35, 238-45, (2003). 14517554

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Keap1kelch-like ECH-associated protein 19Keap1<tm1Mym>targeted mutation 1, Masayuki Yamamoto

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)9neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)9nls

Ordering Information

供与核酸Simian virus 40 large T antigen nuclear localization signal (NLS), E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Keap1 genomic DNA
Research applicationFluorescent Proteins/lacZ System
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
研究成果の公表にあたって寄託者の指定する文献を引用する。Nature Genetics, 35, 238-245 (2003).
寄託後2年間は共著を必要とする共同研究とする。当該リソースを使用した研究結果に基づき特許等の申請を行う場合は、寄託者と別途協議を行う。
Depositor山本　雅之（国立大学法人筑波大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-
Mouse of the Month Oct 2005

BRC mice in Publications

Sakai E, Morita M, Ohuchi M, Kido MA, Fukuma Y, Nishishita K, Okamoto K, Itoh K, Yamamoto M, Tsukuba T.
Effects of deficiency of Kelch-like ECH-associated protein 1 on skeletal organization: a mechanism for diminished nuclear factor of activated T cells cytoplasmic 1 during osteoclastogenesis.
FASEB J 31(9) 4011-4022(2017) 28515152

Chen H, Li J, Li H, Hu Y, Tevebaugh W, Yamamoto M, Que J, Chen X.
Transcript profiling identifies dynamic gene expression patterns and an important role for Nrf2/Keap1 pathway in the developing mouse esophagus.
PLoS One 7(5) e36504(2012) 22567161

Yin Y, Corry KA, Loughran JP, Li J.
Moderate Nrf2 Activation by Genetic Disruption of Keap1 Has Sex-Specific Effects on Bone Mass in Mice.
Sci Rep 10(1) 348(2020) 31941926