Strain Data Sheet

RBRC01386

Strain Information

Image
BRC No.RBRC01386
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;FVB-Tg(ACTA1-cre)AMcle/Rbrc
Former Common nameTransgenic mouse, MuCre-A
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentThe expression construct was created by cloning cDNAs encoding the selected genes and the promoter into a plasmid vector. After verification of the construct by nucleotide sequencing, the transcriptional unit was cleaved out with restriction enzymes, purified with biochemical method and used for microinjection. The offspring were initially examined by PCR genotyping. The in vivo expression was checked by chemical LacZ activity staining after crossing with a Cre-expression detection mouse line (Rosa26-Floxed LacZ). The entire process took from 1999 to 2003.
Strain descriptionMuCreA contains a transcriptional unit consisting of a muscle-specific promoter (alpha-actin promoter) and Cre recombinase gene. This can be used to make an inducible knock-out by crossing with the other GM mice that have Floxed genes. MuCreA is a driver mouse that can remove Floxed genes only in skeletal muscle, but the genes to be removed are marked in the partner lines.
Colony maintenance
ReferencesInt. J. Biol. Sci., 20;6(6):546-555 (2010). 20877696

Health Report

Examination Date / Room / Rack2024/03/11Room:3-2Rack:D
2023/12/11Room:3-2Rack:D
2023/09/11Room:3-2Rack:D
2023/06/13Room:3-2Rack:D
2023/03/13Room:3-2Rack:D
2022/12/12Room:3-2Rack:D
2022/09/12Room:3-2Rack:D
2022/06/13Room:3-2Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcre<Tg(ACTA1-cre)AMcle>transgene insertion A, Ian S. McLennanhuman alpha actin promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Ordering Information

供与核酸human alpha skeletal muscle actin promoter, phage P1 Cre recombinase, SV40 poly A signal
Research applicationCre/loxP system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Int. J. Biol. Sci., 20;6(6):546-555 (2010).
Depositor小石　恭子（University of Otago）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Azhar M, Wang PY, Frugier T, Koishi K, Deng C, Noakes PG, McLennan IS.
Myocardial deletion of Smad4 using a novel α skeletal muscle actin Cre recombinase transgenic mouse causes misalignment of the cardiac outflow tract.
Int J Biol Sci 6(6) 546-55(2010) 20877696

Fujimoto M, Kitamura H.
Application of the colorimetric loop-mediated isothermal amplification (LAMP) technique for genotyping Cre-driver mice.
J Vet Med Sci 84(4) 507-510(2022) 35228407

Uruno A, Yagishita Y, Katsuoka F, Kitajima Y, Nunomiya A, Nagatomi R, Pi J, Biswal SS, Yamamoto M.
Nrf2-Mediated Regulation of Skeletal Muscle Glycogen Metabolism.
Mol Cell Biol 36(11) 1655-72(2016) 27044864

Uruno A, Furusawa Y, Yagishita Y, Fukutomi T, Muramatsu H, Negishi T, Sugawara A, Kensler TW, Yamamoto M.
The Keap1-Nrf2 system prevents onset of diabetes mellitus.
Mol Cell Biol 33(15) 2996-3010(2013) 23716596