Strain Data Sheet

RBRC01385

Strain Information

Image
BRC No.RBRC01385
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/McleRbrc
Former Common nameTransgenic mouse, PTR-H
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentThe expression construct was created by cloning cDNAs encoding the selected genes and the promoter into a plasmid vector. After verification of the construct by nucleotide sequencing, the transcriptional unit was cleaved out with restriction enzymes, purified with biochemical method and used for microinjection. The offspring were initially examined by PCR genotyping and in vitro expression of the marker, EGFP, after transfection with Tet-OFF expression vector (Clontech). The entire process took from 1999 to 2003.
Strain descriptionThe transcriptional unit integrated into PTR-H consists of a dominant negative form of TGF-beta type II receptor gene and EGFP marker under a bidirectional tetracycline-responsive promoter. By crossing with another transgenic mouse that express tetracycline-inducible transcriptional activator, TGF-beta transduction pathway can be disrupted in a conditional manner.
Colony maintenance
ReferencesGenesis 42, 1-5 (2005). 15828000

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tgfbr2transforming growth factor, beta receptor IIUNTgfbr2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNE. coli bidirectional TRE promoter (tetracycline responsive promoter)

Ordering Information

供与核酸TGF-beta type II receptor, Jellyfish GFP cDNA, E. coli bidirectional tetracycline-responsive promoter
Research applicationFluorescent Proteins/lacZ System
Tet system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Frugier, T., Kyoko, K., Matthaei, K.I. and McLennan, I.S. (2005). Transgenic mice carrying a tetracycline-inducible transforming growth factor beta receptor (T_RII). Genesis 42, 1-5.
研究成果の公表にあたって謝辞の表明を必要とする。
Depositor小石　恭子（University of Otago）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data