Strain Data Sheet

RBRC01385

Strain Information

Image
BRC No.RBRC01385
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/McleRbrc
Former Common nameTransgenic mouse, PTR-H
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentThe expression construct was created by cloning cDNAs encoding the selected genes and the promoter into a plasmid vector. After verification of the construct by nucleotide sequencing, the transcriptional unit was cleaved out with restriction enzymes, purified with biochemical method and used for microinjection. The offspring were initially examined by PCR genotyping and in vitro expression of the marker, EGFP, after transfection with Tet-OFF expression vector (Clontech). The entire process took from 1999 to 2003.
Strain descriptionThe transcriptional unit integrated into PTR-H consists of a dominant negative form of TGF-beta type II receptor gene and EGFP marker under a bidirectional tetracycline-responsive promoter. By crossing with another transgenic mouse that express tetracycline-inducible transcriptional activator, TGF-beta transduction pathway can be disrupted in a conditional manner.
Colony maintenance
References
Transgenic mice carrying a tetracycline-inducible, truncated transforming growth factor beta receptor (TbetaRII).
Frugier T, Koishi K, Matthaei K I, McLennan I S
Genesis 42, 1-5 (2005). 15828000

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)UN E. coli bidirectional TRE promoter (tetracycline responsive promoter)
Tgfbr2
MGI:98729		transforming growth factor, beta receptor IIUNTgfbr2
  • colorectal cancer, hereditary nonpolyposis, type 6(MedGEN)

  • esophageal cancer(MedGEN)

  • Loeys-Dietz syndrome 2(MedGEN)
    • more 1 Diseases
  • Loeys-Dietz syndrome(DisGeNET)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNATGF-beta type II receptor, Jellyfish GFP cDNA, E. coli bidirectional tetracycline-responsive promoter
    Research applicationFluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis 42, 1-5 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorKyoko Koishi (University of Otago)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data