Strain Data Sheet

RBRC01363

Strain Information

Image
BRC No.RBRC01363
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(CAG-cat,-Mesp2)1Ysa/YsaRbrc
Former Common nameCAG-CAT-Mesp2
H-2 Haplotype
ES Cell line
Background strainMCH
Appearance
Strain developmentDeveloped by Dr. Yumiko Saga, National Institute of Genetics in 2001. ICR background.
Strain descriptionThe transgene contains CAG promoter and Mesp2 cDNA separated by a floxed chloramphenicol acetyltransferase (CAT) cassette. When the floxed CAT cassette is excised by crossing with Cre mice, Mesp1 is expressed.
Colony maintenance
References
Identification of Epha4 enhancer required for segmental expression and the regulation by Mesp2.
Nakajima Y, Morimoto M, Takahashi Y, Koseki H, Saga Y
Development, 33, 2517-2525 (2006). 16728472

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mesp2
MGI:1096325		mesoderm posterior 2UNMesp2 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
  • spondylocostal dysostosis 1, autosomal recessive(MedGEN)

  • spondylocostal dysostosis 2, autosomal recessive(MedGEN)
    • cat E. coli chloramphenicol acetyltransferase (CAT)UN CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), E. coli chloramphenicol acetyltransferase (CAT) gene, Mesp2 cDNA
    Research applicationCre/loxP system
    Developmental Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 33, 2517-2525 (2006).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP

    BRC mice in Publications

    No Data