Strain Information | |
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Image | |
BRC No. | RBRC01361 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Trp53<tm1Sia>/Rbrc |
Former Common name | C57BL-p53+/- |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | C57BL/6NCrSlc |
Appearance | black [a/a B/B C/C] |
Strain development | 理化学研究所筑波研究所・相沢慎一。TT2 ES細胞(B6CBAF1由来)を用いて作出。京都大学放射線生物研究センター・丹羽大貫(C57BL/6背景、ICR背景およびC3H背景)、大阪大学大学院生命機能研究科・八木 健(DBA/2背景)、新潟大学大学院医歯学総合研究科・木南 凌(MSM背景)らによりコンジェニック育成。 |
Strain description | p53 (Trp53) 遺伝子のノックアウトマウス。p53はアポトーシス、細胞周期、DNA修復機構に関与するガン抑制遺伝子。第2エクソンにhomologous recombinationによりneo遺伝子を挿入。ホモマウスは様々な部位でガンが発生する (3から6ヶ月齢頃までに) 。C57BL/6背景 (RBRC01361) 、ICR背景 (RBRC01348) 、C3H背景 (RBRC00107) 、DBA/2背景 (RBRC01518) およびMSM背景 (RBRC00815) があり。ガンを発症する前であればホモマウスも繁殖可能 (成績は低い) 。C57BL/6背景ではホモマウスのメスはほとんど生まれてこない (MSM背景もおそらく同様) 。 |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NCrSlc] |
References | Oncogene, 8, 3313-3322 (1993). 7504233 |
Health Report | |
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Examination Date / Room / Rack | 2024/01/29Room:3-CRack:E 2024/01/22Room:3-CRack:E 2023/10/23Room:3-CRack:E 2023/07/24Room:3-CRack:E 2023/04/24Room:3-CRack:E 2023/01/23Room:3-CRack:E 2022/10/25Room:3-CRack:E 2022/07/25Room:3-CRack:E 2022/04/25Room:3-CRack:E |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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供与核酸 | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA |
Research application | Cancer Research Cell Biology Research Immunology and Inflammation Research Mouse Models for Human Disease |
提供条件 | 条件を付加する。 研究成果の公表にあたって寄託者の指定する文献を引用する。Oncogene, 8, 3313-3322 (1993). 研究成果の公表にあたっては当該変異マウスのAcc. No.『CDB 0001K』を記すこと。 (Acc. No.に関してはホームページ(https://large.riken.jp/distribution/mutant-list.html)をご覧下さい) 研究成果の公表にあたって発表論文をCDB変異マウス開発チーム mutant.bdr@riken.jp 迄連絡する事。 |
Depositor | 丹羽 太貫(国立大学法人京都大学) |
Strain Status | 生体 凍結胚 凍結精子 |
Strain Availability | 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 生体マウスを1~3ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Breeding characters Mouse of the Month Jun 2005 Genetic Background |
BRC mice in Publications |
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Kobayashi M, Hoshino S, Abe T, Okita N, Tagawa R, Nagai W, Konno R, Suzuki Y, Furuya K, Ishikawa N, Okado H, Oku M, Iwamoto M, Miura Y, Sudo Y, Higami Y. Identification of WWP1 as an obesity-associated E3 ubiquitin ligase with a protective role against oxidative stress in adipocytes. Biochem Biophys Res Commun 508(1) 117-122(2019) 30471861 Saito Y, Onishi N, Takami H, Seishima R, Inoue H, Hirata Y, Kameyama K, Tsuchihashi K, Sugihara E, Uchino S, Ito K, Kawakubo H, Takeuchi H, Kitagawa Y, Saya H, Nagano O. Development of a functional thyroid model based on an organoid culture system. Biochem Biophys Res Commun 497(2) 783-789(2018) 29470983 Okita N, Ishikawa N, Mizunoe Y, Oku M, Nagai W, Suzuki Y, Matsushima S, Mikami K, Okado H, Sasaki T, Higami Y. Inhibitory effect of p53 on mitochondrial content and function during adipogenesis. Biochem Biophys Res Commun 446(1) 91-7(2014) 24565844 Mai HN, Sharma G, Sharma N, Shin EJ, Kim DJ, Pham DT, Trinh QD, Jang CG, Nah SY, Jeong JH, Kim HC. Genetic depletion of p53 attenuates cocaine-induced hepatotoxicity in mice. Biochimie 158 53-61(2019) 30576773 Yamauchi H, Katayama K, Ueno M, Kanemitsu H, Nam C, Mikami T, Saito A, Ishida Y, Uetsuka K, Doi K, Ohmach Y, Nakayama H. Etoposide induces TRP53-dependent apoptosis and TRP53-independent cell cycle arrest in trophoblasts of the developing mouse placenta. Biol Reprod 80(4) 813-22(2009) 19109225 Cai W, Fujita T, Hidaka Y, Jin H, Suita K, Shigeta M, Kiyonari H, Umemura M, Yokoyama U, Sadoshima J, Ishikawa Y. Translationally controlled tumor protein (TCTP) plays a pivotal role in cardiomyocyte survival through a Bnip3-dependent mechanism. Cell Death Dis 10(8) 549(2019) 31320615 Kobatake K, Ikeda KI, Nakata Y, Yamasaki N, Ueda T, Kanai A, Sentani K, Sera Y, Hayashi T, Koizumi M, Miyakawa Y, Inaba T, Sotomaru Y, Kaminuma O, Ichinohe T, Honda ZI, Yasui W, Horie S, Black PC, Matsubara A, Honda H. Kdm6a Deficiency Activates Inflammatory Pathways, Promotes M2 Macrophage Polarization, and Causes Bladder Cancer in Cooperation with p53 Dysfunction. Clin Cancer Res 26(8) 2065-2079(2020) 32047002 Sasaki H, Sugiyama M, Sasaki N. Establishment of renal proximal tubule cell lines derived from the kidney of p53 knockout mice. Cytotechnology 71(1) 45-56(2019) 30603921 Tanikawa C, Zhang YZ, Yamamoto R, Tsuda Y, Tanaka M, Funauchi Y, Mori J, Imoto S, Yamaguchi R, Nakamura Y, Miyano S, Nakagawa H, Matsuda K. The Transcriptional Landscape of p53 Signalling Pathway. EBioMedicine 20 109-119(2017) 28558959 Liu X, Sato N, Yabushita T, Li J, Jia Y, Tamura M, Asada S, Fujino T, Fukushima T, Yonezawa T, Tanaka Y, Fukuyama T, Tsuchiya A, Shikata S, Iwamura H, Kinouchi C, Komatsu K, Yamasaki S, Shibata T, Sasaki AT, Schibler J, Wunderlich M, O'Brien E, Mizukawa B, Mulloy JC, Sugiura Y, Takizawa H, Shibata T, Miyake K, Kitamura T, Goyama S. IMPDH inhibition activates TLR-VCAM1 pathway and suppresses the development of MLL-fusion leukemia. EMBO Mol Med 15(1) e15631(2023) 36453131 Tochitani T, Kanemitsu H, Yamauchi H, Uchida K, Nakayama H. 5-azacytidine, a chemotherapeutic drug, induces TRAIL-mediated apoptosis in mouse thymocytes in vivo. Exp Toxicol Pathol 63(3) 237-42(2011) 20092982 Nam C, Doi K, Nakayama H. Etoposide induces G2/M arrest and apoptosis in neural progenitor cells via DNA damage and an ATM/p53-related pathway. Histol Histopathol 25(4) 485-93(2010) 20183801 Ohkawa Y, Momota H, Kato A, Hashimoto N, Tsuda Y, Kotani N, Honke K, Suzumura A, Furukawa K, Ohmi Y, Natsume A, Wakabayashi T, Furukawa K. Ganglioside GD3 Enhances Invasiveness of Gliomas by Forming a Complex with Platelet-derived Growth Factor Receptor α and Yes Kinase. J Biol Chem 290(26) 16043-58(2015) 25940087 Tobisawa Y, Imai Y, Fukuda M, Kawashima H. Sulfation of colonic mucins by N-acetylglucosamine 6-O-sulfotransferase-2 and its protective function in experimental colitis in mice. J Biol Chem 285(9) 6750-60(2010) 20018871 Okahashi N, Kohno S, Kitajima S, Matsuda F, Takahashi C, Shimizu H. Metabolic characterization of cultured mammalian cells by mass balance analysis, tracer labeling experiments and computer-aided simulations. J Biosci Bioeng 120(6) 725-31(2015) 25936961 Nagashima T, Ninomiya T, Nakamura Y, Nishimura S, Ohashi A, Aoki J, Mizoguchi T, Tonogi M, Takahashi T. p53 deficiency promotes bone regeneration by functional regulation of mesenchymal stromal cells and osteoblasts. J Bone Miner Metab 40(3) 434-447(2022) 35195777 Kawashima H. Roles of GlcNAc-6-O-sulfotransferases in lymphoid and nonlymphoid tissues. Methods Enzymol 479 243-56(2010) 20816170 Shin EJ, Nam Y, Lee JW, Nguyen PT, Yoo JE, Tran TV, Jeong JH, Jang CG, Oh YJ, Youdim MBH, Lee PH, Nabeshima T, Kim HC. N-Methyl, N-propynyl-2-phenylethylamine (MPPE), a Selegiline Analog, Attenuates MPTP-induced Dopaminergic Toxicity with Guaranteed Behavioral Safety: Involvement of Inhibitions of Mitochondrial Oxidative Burdens and p53 Gene-elicited Pro-apoptotic Change. Mol Neurobiol 53(9) 6251-6269(2016) 26563498 Zhang P, Ohkawa Y, Yamamoto S, Momota H, Kato A, Kaneko K, Natsume A, Farhana Y, Ohmi Y, Okajima T, Bhuiyan RH, Wakabayashi T, Furukawa K, Furukawa K. St8sia1-deficiency in mice alters tumor environments of gliomas, leading to reduced disease severity. Nagoya J Med Sci 83(3) 535-549(2021) 34552288 Saito Y, Chikenji TS, Matsumura T, Nakano M, Fujimiya M. Exercise enhances skeletal muscle regeneration by promoting senescence in fibro-adipogenic progenitors. Nat Commun 11(1) 889(2020) 32060352 Hayashi Y, Goyama S, Liu X, Tamura M, Asada S, Tanaka Y, Fukuyama T, Wunderlich M, O'Brien E, Mizukawa B, Yamazaki S, Matsumoto A, Yamasaki S, Shibata T, Matsuda K, Sashida G, Takizawa H, Kitamura T. Antitumor immunity augments the therapeutic effects of p53 activation on acute myeloid leukemia. Nat Commun 10(1) 4869(2019) 31653912 Kanemitsu H, Yamauchi H, Komatsu M, Yamamoto S, Okazaki S, Uchida K, Nakayama H. 6-mercaptopurine (6-MP) induces p53-mediated apoptosis of neural progenitor cells in the developing fetal rodent brain. Neurotoxicol Teratol 31(4) 198-202(2009) 19281843 Tokumasu R, Yasuhara R, Kang S, Funatsu T, Mishima K. Transcription factor FoxO1 regulates myoepithelial cell diversity and growth. Sci Rep 14(1) 1069(2024) 38212454 Tamura M, Yonezawa T, Liu X, Asada S, Hayashi Y, Fukuyama T, Tanaka Y, Kitamura T, Goyama S. Opposing effects of acute versus chronic inhibition of p53 on decitabine's efficacy in myeloid neoplasms. Sci Rep 9(1) 8171(2019) 31160638 Kanatsu-Shinohara M, Naoki H, Tanaka T, Tatehana M, Kikkawa T, Osumi N, Shinohara T. Regulation of male germline transmission patterns by the Trp53-Cdkn1a pathway. Stem Cell Reports 17(9) 1924-1941(2022) 35931081 Kitajima S, Kohno S, Kondoh A, Sasaki N, Nishimoto Y, Li F, Abdallah Mohammed MS, Muranaka H, Nagatani N, Suzuki M, Kido Y, Takahashi C. Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts. Stem Cells 33(5) 1657-69(2015) 25694388 |