Strain Data Sheet

RBRC01354

Strain Information

Image
BRC No.RBRC01354
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Pvalb-IL2RA/EGFP)2Kyo
Former Common namePA2
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Masaaki Hazama and Shigetada Nakanishi in 2002.
Strain descriptionB6.D2-Tg(Pvalb-IL2RA/EGFP)2Kyo. The calcium-binding protein, parvalbumin, Pvalb is expressed in fast-twitch fibers in skeletal muscles and a subpopulation of inhibitory neurons in the CNS. These mice express human interleuikin-2 receptor alpha-subunit (IL2RA)-GFP under the control of Pvalb gene promoter.
Colony maintenanceSibling Mating
References
Different regulatory sequences are required for parvalbumin gene expression in skeletal muscles and neuronal cells of transgenic mice.
Hazama M, Watanabe D, Suzuki M, Mizoguchi A, Pastan I, Nakanishi S
Mol. Brain Res., 100, 53-66 (2002). 12008021

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UN
IL2RAInterleukin-2 receptor α-subunit (human)UNIL2RAmouse Parvalbumin promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Parvalbumin promoter, human IL-2R cDNA, jellyfish GFP cDNA, SV40 poly A
    Research applicationFluorescent Proteins/lacZ System
    General Purpose
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Brain. Res., 100, 53-66 (2002). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorShigetada Nakanishi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data