Strain Data Sheet

RBRC01353

Strain Information

Image
BRC No.RBRC01353
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Grm6-lacZ)35Kyo- Grm6<tm1Kyo>
Former Common namemGluR6-/-/lacZ+
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain development京都大学医学部・中西重忠先生。Tg：B6D2F1受精卵へトランスジーンのインジェクションにより作出。KO：CCE/EK.CCE ES細胞（129S/SvEv-Gpi1<c>由来）を用いて作出。C57BL/6セミコンジェニック。
Strain descriptionGrm6-lacZトランスジェニック、Grm6遺伝子ノックアウトのダブルミュータントマウス。
Colony maintenanceTg: Carrier x Carrier; KO: Homozygote x Homozygote
References
Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
M Masu, H Iwakabe, Y Tagawa, T Miyoshi, M Yamashita, Y Fukuda, H Sasaki, K Hiroi, Y Nakamura, R Shigemoto
Cell, 80, 757-765 (1995). 7889569

The mGluR6 5' upstream transgene sequence directs a cell-specific and developmentally regulated expression in retinal rod and ON-type cone bipolar cells.
Y Ueda, H Iwakabe, M Masu, M Suzuki, S Nakanishi
J. Neurosci., 17, 3014-3023 (1997). 9096137

Immunohistological studies of metabotropic glutamate receptor subtype 6-deficient mice show no abnormality of retinal cell organization and ganglion cell maturation.
Y Tagawa, H Sawai, Y Ueda, M Tauchi, S Nakanishi
J. Neurosci., 19, 2568-2579 (1999). 10087070

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grm6
MGI:1351343		glutamate receptor, metabotropic 611Grm6<tm1Nak>
MGI:1857985		targeted mutation 1, Shigetada Nakanishi
  • congenital stationary night blindness 1B(DisGeNET, MedGEN)

  • congenital stationary night blindness(DisGeNET)
    • lacZ beta-galactosidase (E. coli)11 mouse mGluR6 promoter
    neo neomycin resistance gene (E. coli)11 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal eye electrophysiology (MP:0005551)

  • abnormal synaptic transmission (MP:0003635)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse mGluR6 promoter, E. coli lacZ, SV40 poly A, E. coli neo, herpes simplex virus thymidine kinase promoter (HSV tk promoter), mouse grm6 genomic DNA
    Research applicationGeneral Purpose
    Neurobiology Research
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。J. Neurosci., 19, 2568-2579 (1999). J. Neurosci., 17, 3014-3023 (1997). Cell, 80, 757-765 (1995).
    Depositor中西　重忠（国立大学法人京都大学）
    Strain Status凍結胚のアイコン凍結胚
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data