Strain Data Sheet


Strain Information

BRC No.RBRC01353
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Grm6-lacZ)35Kyo- Grm6<tm1Kyo>
Former Common namemGluR6-/-/lacZ+
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Strain developmentDeveloped by Shigetada Nakanishi, Kyoto University Faculty of Medicine. Tg: the transgene was injected into the pronuclei of B6D2F1 fertilized eggs. KO: CCE/EK.CCE ES cells derived from 129S/SvEv-Gpi1<c> were used to generate the mutant mice. C57BL/6 semicongenic.
Strain descriptionDouble mutant mice with transgene and knockout allele. Grm6 (glutamate receptor, metabotropic 6) gene is suggested to be responsible for ON responses in both the rod and cone systems. The transgene is mGluR6 gene fused to the beta-galactosidase reporter gene. This strain is useful for investigating the promoter function of the cell-specific and developmentally regulated expression of mGluR6.
Colony maintenanceTg: Carrier x Carrier; KO: Homozygote x Homozygote
ReferencesJ. Neurosci., 19, 2568-2579 (1999). 10087070
J. Neurosci., 17, 3014-3023 (1997). 9096137
Cell, 80, 757-765 (1995). 7889569

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Grm6glutamate receptor, metabotropic 611Grm6<tm1Nak>targeted mutation 1, Shigetada NakanishimGluR6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)11mouse mGluR6 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAmouse mGluR6 promoter, E. coli lacZ, SV40 poly A, E. coli neo, herpes simplex virus thymidine kinase promoter (HSV tk promoter), mouse grm6 genomic DNA
Research applicationGeneral Purpose
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 19, 2568-2579 (1999). J. Neurosci., 17, 3014-3023 (1997). Cell, 80, 757-765 (1995).
DepositorShigetada Nakanishi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data