Strain Data Sheet

RBRC01353

Strain Information

Image
BRC No.RBRC01353
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Grm6-lacZ)35Kyo- Grm6<tm1Kyo>
Former Common namemGluR6-/-/lacZ+
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Shigetada Nakanishi, Kyoto University Faculty of Medicine. Tg: the transgene was injected into the pronuclei of B6D2F1 fertilized eggs. KO: CCE/EK.CCE ES cells derived from 129S/SvEv-Gpi1<c> were used to generate the mutant mice. C57BL/6 semicongenic.
Strain descriptionDouble mutant mice with transgene and knockout allele. Grm6 (glutamate receptor, metabotropic 6) gene is suggested to be responsible for ON responses in both the rod and cone systems. The transgene is mGluR6 gene fused to the beta-galactosidase reporter gene. This strain is useful for investigating the promoter function of the cell-specific and developmentally regulated expression of mGluR6.
Colony maintenanceTg: Carrier x Carrier; KO: Homozygote x Homozygote
References
Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Masu M, Iwakabe H, Tagawa Y, Miyoshi T, Yamashita M, Fukuda Y, Sasaki H, Hiroi K, Nakamura Y, Shigemoto R, et a l
Cell, 80, 757-765 (1995). 7889569

The mGluR6 5' upstream transgene sequence directs a cell-specific and developmentally regulated expression in retinal rod and ON-type cone bipolar cells.
Ueda Y, Iwakabe H, Masu M, Suzuki M, Nakanishi S
J. Neurosci., 17, 3014-3023 (1997). 9096137

Immunohistological studies of metabotropic glutamate receptor subtype 6-deficient mice show no abnormality of retinal cell organization and ganglion cell maturation.
Tagawa Y, Sawai H, Ueda Y, Tauchi M, Nakanishi S
J. Neurosci., 19, 2568-2579 (1999). 10087070

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grm6glutamate receptor, metabotropic 611Grm6targeted mutation 1, Shigetada Nakanishi
  • congenital stationary night blindness 1B(DisGeNET, MedGEN)
    • lacZbeta-galactosidase (E. coli)11mouse mGluR6 promoter
  • congenital stationary night blindness 1B(DisGeNET, MedGEN)
    • neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • congenital stationary night blindness 1B(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal eye electrophysiology(MP:0005551)

  • abnormal synaptic transmission(MP:0003635)

  • behavior/neurological phenotype(MP:0005386)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse mGluR6 promoter, E. coli lacZ, SV40 poly A, E. coli neo, herpes simplex virus thymidine kinase promoter (HSV tk promoter), mouse grm6 genomic DNA
    Research applicationGeneral Purpose
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 19, 2568-2579 (1999). J. Neurosci., 17, 3014-3023 (1997). Cell, 80, 757-765 (1995).
    DepositorShigetada Nakanishi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data