Strain Data Sheet

RBRC01351

Strain Information

Image
BRC No.RBRC01351
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S-Grm2<tm1Nak>/NakRbrc
Former Common nameC57BL/6-Grm2 tm (Nov.2011), GluR2 KO, mGluR2 KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentC57BL/6系統（substrain不明）で8世代、その後C57BL/6N（チャールズリバー）で12世代戻し交配を行った。
Strain descriptionmGluR2ノックアウトマウス。コカインへの依存行動が亢進している。行動の異常がある
Colony maintenance繁殖効率A特になし
References
Enhanced cocaine responsiveness and impaired motor coordination in metabotropic glutamate receptor subtype 2 knockout mice.
Yosuke Morishima, Tsuyoshi Miyakawa, Tomoyuki Furuyashiki, Yasuhiro Tanaka, Hiroshi Mizuma, Shigetada Nakanishi
Proc. Natl. Acad. Sci. USA, 102, 4170-4175 (1996). 15753323

Impairment of hippocampal mossy fiber LTD in mice lacking mGluR2.
M Yokoi, K Kobayashi, T Manabe, T Takahashi, I Sakaguchi, G Katsuura, R Shigemoto, H Ohishi, S Nomura, K Nakamura, K Nakao, M Katsuki, S Nakanishi
Science, 273, 645-647 (1996). 8662555

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grm2
MGI:1351339		glutamate receptor, metabotropic 29Grm2<tm1Nak> targeted mutation 1, Shigetada Nakanishi
neo neomycin resistance gene (E. coli)9 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal GABAergic neuron physiology (MP:0011987)

  • abnormal excitatory postsynaptic potential (MP:0002912)

  • abnormal motor capabilities/coordination/movement (MP:0002066)

  • cocaine preference (MP:0001988)

  • decreased susceptibility to neuronal excitotoxicity (MP:0008236)
    • more 4 phenotypes
  • impaired coordination (MP:0001405)

  • increased dopamine level (MP:0001906)

  • increased locomotor activity (MP:0031391)

  • reduced long-term depression (MP:0001475)
    • Detailed phenotype data

    Ordering Information

    供与核酸herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse glutamate receptor metabotropic 2 genomic DNA
    Research applicationNeurobiology Research
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Science, 273, 645-647 (1996). Proc Natl Acad Sci USA, 102, 4170-4175 (2005). 研究成果の公表にあたって謝辞の表明を必要とする。
    Depositor中西　重忠（国立大学法人京都大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Kim H, Lee Y, Park JY, Kim JE, Kim TK, Choi J, Lee JE, Lee EH, Kim D, Kim KS, Han PL.
    Loss of Adenylyl Cyclase Type-5 in the Dorsal Striatum Produces Autistic-Like Behaviors.
    Mol Neurobiol 54(10) 7994-8008(2017) 27878759
    Moreno JL, Miranda-Azpiazu P, García-Bea A, Younkin J, Cui M, Kozlenkov A, Ben-Ezra A, Voloudakis G, Fakira AK, Baki L, Ge Y, Georgakopoulos A, Morón JA, Milligan G, López-Giménez JF, Robakis NK, Logothetis DE, Meana JJ, González-Maeso J.
    Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia.
    Sci Signal 9(410) ra5(2016) 26758213
    Moreno JL, Holloway T, Rayannavar V, Sealfon SC, González-Maeso J.
    Chronic treatment with LY341495 decreases 5-HT(2A) receptor binding and hallucinogenic effects of LSD in mice.
    Neurosci Lett 536 69-73(2013) 23333599
    Moreno JL, Muguruza C, Umali A, Mortillo S, Holloway T, Pilar-Cuéllar F, Mocci G, Seto J, Callado LF, Neve RL, Milligan G, Sealfon SC, López-Giménez JF, Meana JJ, Benson DL, González-Maeso J.
    Identification of three residues essential for 5-hydroxytryptamine 2A-metabotropic glutamate 2 (5-HT2A·mGlu2) receptor heteromerization and its psychoactive behavioral function.
    J Biol Chem 287(53) 44301-19(2012) 23129762
    Fribourg M, Moreno JL, Holloway T, Provasi D, Baki L, Mahajan R, Park G, Adney SK, Hatcher C, Eltit JM, Ruta JD, Albizu L, Li Z, Umali A, Shim J, Fabiato A, MacKerell AD Jr, Brezina V, Sealfon SC, Filizola M, González-Maeso J, Logothetis DE.
    Decoding the signaling of a GPCR heteromeric complex reveals a unifying mechanism of action of antipsychotic drugs.
    Cell 147(5) 1011-23(2011) 22118459
    Moreno JL, Holloway T, Albizu L, Sealfon SC, González-Maeso J.
    Metabotropic glutamate mGlu2 receptor is necessary for the pharmacological and behavioral effects induced by hallucinogenic 5-HT2A receptor agonists.
    Neurosci Lett 493(3) 76-9(2011) 21276828