Strain Data Sheet

RBRC01348

Strain Information

Image
BRC No.RBRC01348
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Trp53<tm1Sia>/Rbrc
Former Common nameICR-p53+/-
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearancealbino [c/c]
Strain development理化学研究所筑波研究所・相沢慎一。TT2 ES細胞（B6CBAF1由来）を用いて作出。京都大学放射線生物研究センター・丹羽大貫（C57BL/6背景、ICR背景およびC3H背景）、大阪大学大学院生命機能研究科・八木　健（DBA/2背景）、新潟大学大学院医歯学総合研究科・木南　凌（MSM背景）らによりコンジェニック育成。
Strain descriptionp53 (Trp53) 遺伝子のノックアウトマウス。p53はアポトーシス、細胞周期、DNA修復機構に関与するガン抑制遺伝子。第2エクソンにhomologous recombinationによりneo遺伝子を挿入。ホモマウスは様々な部位でガンが発生する (3から6ヶ月齢頃までに) 。C57BL/6背景 (RBRC01361) 、ICR背景 (RBRC01348) 、C3H背景 (RBRC00107) 、DBA/2背景 (RBRC01518) およびMSM背景 (RBRC00815) があり。ガンを発症する前であればホモマウスも繁殖可能 (成績は低い) 。C57BL/6背景ではホモマウスのメスはほとんど生まれてこない (MSM背景もおそらく同様) 。
Colony maintenanceHeterozygote x Wild-type [or Crossing to Slc:ICR]
References
Enhanced proliferative potential in culture of cells from p53-deficient mice.
T Tsukada, Y Tomooka, S Takai, Y Ueda, S Nishikawa, T Yagi, T Tokunaga, N Takeda, Y Suda, S Abe
Oncogene, 8, 3313-3322 (1993). 7504233

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Trp53
MGI:98834		transformation related protein 5311Trp53<tm1Sia>
MGI:1926340		targeted mutation 1, Shinichi Aizawa
  • adrenal cortex carcinoma(DisGeNET)

  • adrenocortical carcinoma, hereditary(MedGEN)

  • B-cell chronic lymphocytic leukemia(DisGeNET)
    • more 20 Diseases
  • basal cell carcinoma, susceptibility to, 7(MedGEN)

  • bone marrow failure syndrome 5(MedGEN)

  • bone osteosarcoma(MedGEN)

  • breast cancer(DisGeNET)

  • breast carcinoma(DisGeNET)

  • choroid plexus papilloma(MedGEN)

  • colorectal cancer(MedGEN)

  • exocrine pancreatic carcinoma(MedGEN)

  • glioma susceptibility 1(MedGEN)

  • glioma(DisGeNET)

  • hepatocellular carcinoma(DisGeNET, MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • Li-Fraumeni syndrome(DisGeNET)

  • lung cancer(DisGeNET)

  • lung neoplasm(DisGeNET)

  • nasopharyngeal carcinoma(MedGEN)

  • obsolete Li-Fraumeni syndrome 1(MedGEN)

  • osteosarcoma(DisGeNET)

  • pancreatic neoplasm(DisGeNET)

  • sarcoma(DisGeNET)
    • neo neomycin resistance gene (E. coli)11 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal cell proliferation (MP:0000350)

  • decreased cellular sensitivity to ultraviolet irradiation (MP:0008411)

  • increased T cell derived lymphoma incidence (MP:0002024)

  • increased sensitivity to induced cell death (MP:0008943)

  • increased tumor incidence (MP:0002020)
    • Detailed phenotype data

    Ordering Information

    供与核酸herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA
    Research applicationCancer Research
    Cell Biology Research
    Immunology and Inflammation Research
    Mouse Models for Human Disease
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Oncogene, 8, 3313-3322 (1993). 研究成果の公表にあたっては当該変異マウスのAcc. No.『CDB 0001K』を記すこと。
    (Acc. No.に関してはホームページ(https://large.riken.jp/distribution/mutant-list.html)をご覧下さい)
    研究成果の公表にあたって発表論文をCDB変異マウス開発チーム mutant.bdr@riken.jp 迄連絡する事。
    Depositor丹羽　太貫（国立大学法人京都大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jun 2005

    BRC mice in Publications

    Nishiyama Y, Sonoyama Y, Wang B, Ghosh S, Krishnan P V S, Sasatani M, Shimokawa T, Aoki S, Morita A.
    Sodium orthovanadate alleviates lethal effect of total-body irradiation in mice without impairing recovery of male reproductive function.
    Radiat Environ Biophys (2025) 40928532
    Okuda A, Kurokawa S, Takehashi M, Maeda A, Fukuda K, Kubo Y, Nogusa H, Takatani-Nakase T, Okuda S, Ueda K, Tanaka S.
    Poly(ADP-ribose) polymerase inhibitors activate the p53 signaling pathway in neural stem/progenitor cells.
    BMC Neurosci 18(1) 14(2017) 28095779
    Maruzuru Y, Koyanagi N, Takemura N, Uematsu S, Matsubara D, Suzuki Y, Arii J, Kato A, Kawaguchi Y.
    p53 Is a Host Cell Regulator during Herpes Simplex Encephalitis.
    J Virol 90(15) 6738-6745(2016) 27170756