Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC01345 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.129P2-Emx1<tm1.1(cre)Ito>/ItoRbrc |
| Former Common name | Emx1-Cre KI Δneo |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | C57BL/6JJcl |
| Appearance | Black [a/a B/B C/C] |
| Strain development | 理化学研究所脳科学総合研究センター・岩里琢治、糸原重美。E14 ES細胞を用いて作出。C57BL/6背景。 |
| Strain description | Emx1-creノックインマウス。Cre遺伝子とPGK-neoカセットがEmx1遺伝子の翻訳開始コドンATGの前に挿入されているマウス (neo型) (RBRC00808) とPGK-neoカセットを除去したマウス (delta neo型) (RBRC01345) 。大脳皮質、海馬、嗅球の興奮性神経細胞特異的にCre組換え酵素を発現する。delta neo型マウス (RBRC01345) では、neo型で観察されたectopic expressionが消失しており、内在性遺伝子の発現特性をより反映している。ホモマウスは生存性、繁殖性ともにあり。 |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
| References | Cortex-restricted disruption of NMDAR1 impairs neuronal patterns in the barrel cortex. Iwasato T, Datwani A, Wolf A M, Nishiyama H, Taguchi Y, Tonegawa S, Knopfel T, Erzurumlu R S, Itohara S Nature, 406, 726-731 (2000). 10963597Dorsal telencephalon-specific expression of Cre recombinase in PAC transgenic mice. Iwasato T, Nomura R, Ando R, Ikeda T, Tanaka M, Itohara S Genesis, 38, 130-138 (2004). 15048810 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/11/10Room:3-ARack:L囮検査 2025/09/01Room:3-ARack:L囮検査 2025/06/16Room:3-ARack:L囮検査 2025/04/07Room:3-ARack:L囮検査 2025/01/07Room:3-ARack:L囮検査 2024/10/07Room:3-ARack:L囮検査 2024/07/08Room:3-ARack:L囮検査 2024/04/08Room:3-ARack:L囮検査 |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Emx1 MGI:95387 | empty spiracles homeobox 1 | 6 | Emx1<tm1.1(cre)Ito> MGI:3762664 | targeted mutation 1.1, Shigeyoshi Itohara | |||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| cre | Phage P1 Cre recombinase | 6 | cre | ||||
| nls | SV40 large T antigen nuclear localization signal (NLS) | 6 | nls | ||||
| SV40 polyA | 6 | ||||||
Phenotype | |
|---|---|
| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| 供与核酸 | Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) site, mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) genomic DNA |
| Research application | Cell Biology Research Cre/loxP system FLP/frt system Neurobiology Research Cre driver |
| 提供条件 | 研究成果の公表にあたって寄託者の指定する文献を引用する。Genesis, 38, 130-138 (2004). |
| Depositor | 糸原 重美(独立行政法人理化学研究所) |
| Strain Status | 生体 凍結胚 凍結精子 |
| Strain Availability | 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 生体マウスを3~6ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month June 2006 |
BRC mice in Publications |
|---|
Nagashima S, Ito N, Kobayashi R, Shiiba I, Shimura H, Fukuda T, Hagihara H, Miyakawa T, Inatome R, Yanagi S. Forebrain-specific deficiency of the GTPase CRAG/Centaurin-γ3 leads to immature dentate gyri and hyperactivity in mice. J Biol Chem 296 100620(2021) 33811862 |
Mizusawa A, Watanabe A, Yamada M, Kamei R, Shimomura Y, Kitaura Y. BDK Deficiency in Cerebral Cortex Neurons Causes Neurological Abnormalities and Affects Endurance Capacity. Nutrients 12(8) (2020) 32751134 |
Sugimoto J, Tanaka M, Sugiyama K, Ito Y, Aizawa H, Soma M, Shimizu T, Mitani A, Tanaka K. Region-specific deletions of the glutamate transporter GLT1 differentially affect seizure activity and neurodegeneration in mice. Glia 66(4) 777-788(2018) 29214672 |
Sato T, Ishikawa M, Mochizuki M, Ohta M, Ohkura M, Nakai J, Takamatsu N, Yoshioka K. JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration. Cell Death Differ 22(8) 1260-74(2015) 25571974 |
Takagi T, Nishizaki Y, Matsui F, Wakamatsu N, Higashi Y. De novo inbred heterozygous Zeb2/Sip1 mutant mice uniquely generated by germ-line conditional knockout exhibit craniofacial, callosal and behavioral defects associated with Mowat-Wilson syndrome. Hum Mol Genet 24(22) 6390-402(2015) 26319231 |
Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A. Simultaneous detection of multiple transgenes for genetically-modified mouse strains. Exp Anim 58(4) 437-42(2009) 19654444 |