Strain Information | |
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Image | |
BRC No. | RBRC01345 |
Type | Targeted Mutation Congenic![]() |
Species | Mus musculus |
Strain name | B6.129P2-Emx1<tm1.1(cre)Ito>/ItoRbrc |
Former Common name | Emx1-Cre KI Δneo |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C57BL/6JJcl |
Appearance | Black [a/a B/B C/C] |
Strain development | 理化学研究所脳科学総合研究センター・岩里琢治、糸原重美。E14 ES細胞を用いて作出。C57BL/6背景。 |
Strain description | Emx1-creノックインマウス。Cre遺伝子とPGK-neoカセットがEmx1遺伝子の翻訳開始コドンATGの前に挿入されているマウス (neo型) (RBRC00808) とPGK-neoカセットを除去したマウス (delta neo型) (RBRC01345) 。大脳皮質、海馬、嗅球の興奮性神経細胞特異的にCre組換え酵素を発現する。delta neo型マウス (RBRC01345) では、neo型で観察されたectopic expressionが消失しており、内在性遺伝子の発現特性をより反映している。ホモマウスは生存性、繁殖性ともにあり。 |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Cortex-restricted disruption of NMDAR1 impairs neuronal patterns in the barrel cortex. Iwasato T, Datwani A, Wolf A M, Nishiyama H, Taguchi Y, Tonegawa S, Knopfel T, Erzurumlu R S, Itohara S Nature, 406, 726-731 (2000). 10963597Dorsal telencephalon-specific expression of Cre recombinase in PAC transgenic mice. Iwasato T, Nomura R, Ando R, Ikeda T, Tanaka M, Itohara S Genesis, 38, 130-138 (2004). 15048810 |
Health Report | |
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Examination Date / Room / Rack | 2024/10/07Room:3-ARack:L 2024/07/08Room:3-ARack:L 2024/04/08Room:3-ARack:L 2024/01/09Room:3-ARack:L 2023/10/10Room:3-ARack:L 2023/07/10Room:3-ARack:L 2023/04/10Room:3-ARack:L |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Emx1 MGI:95387 | empty spiracles homeobox 1 | 6 | Emx1<tm1.1(cre)Ito> MGI:3762664 | targeted mutation 1.1, Shigeyoshi Itohara | |||
Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
cre | Phage P1 Cre recombinase | 6 | cre | ||||
nls | SV40 large T antigen nuclear localization signal (NLS) | 6 | nls | ||||
SV40 polyA | 6 |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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供与核酸 | Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) site, mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) genomic DNA |
Research application | Cell Biology Research Cre/loxP system FLP/frt system Neurobiology Research |
提供条件 | 研究成果の公表にあたって寄託者の指定する文献を引用する。Genesis, 38, 130-138 (2004). |
Depositor | 糸原 重美(独立行政法人理化学研究所) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 生体マウスを3~6ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month June 2006 |
BRC mice in Publications |
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Nagashima S, Ito N, Kobayashi R, Shiiba I, Shimura H, Fukuda T, Hagihara H, Miyakawa T, Inatome R, Yanagi S. Forebrain-specific deficiency of the GTPase CRAG/Centaurin-γ3 leads to immature dentate gyri and hyperactivity in mice. J Biol Chem 296 100620(2021) 33811862 |
Mizusawa A, Watanabe A, Yamada M, Kamei R, Shimomura Y, Kitaura Y. BDK Deficiency in Cerebral Cortex Neurons Causes Neurological Abnormalities and Affects Endurance Capacity. Nutrients 12(8) (2020) 32751134 |
Sugimoto J, Tanaka M, Sugiyama K, Ito Y, Aizawa H, Soma M, Shimizu T, Mitani A, Tanaka K. Region-specific deletions of the glutamate transporter GLT1 differentially affect seizure activity and neurodegeneration in mice. Glia 66(4) 777-788(2018) 29214672 |
Sato T, Ishikawa M, Mochizuki M, Ohta M, Ohkura M, Nakai J, Takamatsu N, Yoshioka K. JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration. Cell Death Differ 22(8) 1260-74(2015) 25571974 |
Takagi T, Nishizaki Y, Matsui F, Wakamatsu N, Higashi Y. De novo inbred heterozygous Zeb2/Sip1 mutant mice uniquely generated by germ-line conditional knockout exhibit craniofacial, callosal and behavioral defects associated with Mowat-Wilson syndrome. Hum Mol Genet 24(22) 6390-402(2015) 26319231 |
Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A. Simultaneous detection of multiple transgenes for genetically-modified mouse strains. Exp Anim 58(4) 437-42(2009) 19654444 |