Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC01345 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.129P2-Emx1<tm1.1(cre)Ito>/ItoRbrc |
| Former Common name | Emx1-Cre KI Δneo |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | C57BL/6JJcl |
| Appearance | Black [a/a B/B C/C] |
| Strain development | 理化学研究所脳科学総合研究センター・岩里琢治、糸原重美。E14 ES細胞を用いて作出。C57BL/6背景。 |
| Strain description | Emx1-creノックインマウス。Cre遺伝子とPGK-neoカセットがEmx1遺伝子の翻訳開始コドンATGの前に挿入されているマウス (neo型) (RBRC00808) とPGK-neoカセットを除去したマウス (delta neo型) (RBRC01345) 。大脳皮質、海馬、嗅球の興奮性神経細胞特異的にCre組換え酵素を発現する。delta neo型マウス (RBRC01345) では、neo型で観察されたectopic expressionが消失しており、内在性遺伝子の発現特性をより反映している。ホモマウスは生存性、繁殖性ともにあり。 |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
| References | Cortex-restricted disruption of NMDAR1 impairs neuronal patterns in the barrel cortex. T Iwasato, A Datwani, A M Wolf, H Nishiyama, Y Taguchi, S Tonegawa, T Knöpfel, R S Erzurumlu, S Itohara Nature, 406, 726-731 (2000). 10963597Dorsal telencephalon-specific expression of Cre recombinase in PAC transgenic mice. Takuji Iwasato, Ryochi Nomura, Reiko Ando, Toshio Ikeda, Mika Tanaka, Shigeyoshi Itohara Genesis, 38, 130-138 (2004). 15048810 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/11/10Room:3-ARack:L囮検査 2025/09/01Room:3-ARack:L囮検査 2025/06/16Room:3-ARack:L囮検査 2025/04/07Room:3-ARack:L囮検査 2025/01/07Room:3-ARack:L囮検査 2024/10/07Room:3-ARack:L囮検査 2024/07/08Room:3-ARack:L囮検査 |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Emx1 MGI:95387 | empty spiracles homeobox 1 | 6 | Emx1<tm1.1(cre)Ito> MGI:3762664 | targeted mutation 1.1, Shigeyoshi Itohara | |||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| cre | Phage P1 Cre recombinase | 6 | cre | ||||
| nls | SV40 large T antigen nuclear localization signal (NLS) | 6 | nls | ||||
| SV40 polyA | 6 | ||||||
Phenotype | |
|---|---|
| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| 供与核酸 | Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) site, mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) genomic DNA |
| Research application | Cell Biology Research Cre/loxP system FLP/frt system Neurobiology Research Cre driver |
| 提供条件 | 研究成果の公表にあたって寄託者の指定する文献を引用する。Genesis, 38, 130-138 (2004). |
| Depositor | 糸原 重美(独立行政法人理化学研究所) |
| Strain Status | 生体 凍結胚 凍結精子 |
| Strain Availability | 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 生体マウスを3~6ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month June 2006 |
BRC mice in Publications |
|---|
Nakashiba T, Sugiyama T, Iwano S, Iwama M, Yoshiki A, Miyawaki A, Abe K. Assessing leaky expression of Cre-dependent DNA constructs in the mouse genome using sensitive bioluminescent reporters. Cell Rep Methods 101369(2026) 41916303 |
Nakashiba T, Ogoh K, Iwano S, Sugiyama T, Mizuno-Iijima S, Nakashima K, Mizuno S, Sugiyama F, Yoshiki A, Miyawaki A, Abe K. Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging. Lab Anim (NY) (2023) 37679611 |
Nagashima S, Ito N, Kobayashi R, Shiiba I, Shimura H, Fukuda T, Hagihara H, Miyakawa T, Inatome R, Yanagi S. Forebrain-specific deficiency of the GTPase CRAG/Centaurin-γ3 leads to immature dentate gyri and hyperactivity in mice. J Biol Chem 296 100620(2021) 33811862 |
Mizusawa A, Watanabe A, Yamada M, Kamei R, Shimomura Y, Kitaura Y. BDK Deficiency in Cerebral Cortex Neurons Causes Neurological Abnormalities and Affects Endurance Capacity. Nutrients 12(8) (2020) 32751134 |
Sugimoto J, Tanaka M, Sugiyama K, Ito Y, Aizawa H, Soma M, Shimizu T, Mitani A, Tanaka K. Region-specific deletions of the glutamate transporter GLT1 differentially affect seizure activity and neurodegeneration in mice. Glia 66(4) 777-788(2018) 29214672 |
Sato T, Ishikawa M, Mochizuki M, Ohta M, Ohkura M, Nakai J, Takamatsu N, Yoshioka K. JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration. Cell Death Differ 22(8) 1260-74(2015) 25571974 |
Takagi T, Nishizaki Y, Matsui F, Wakamatsu N, Higashi Y. De novo inbred heterozygous Zeb2/Sip1 mutant mice uniquely generated by germ-line conditional knockout exhibit craniofacial, callosal and behavioral defects associated with Mowat-Wilson syndrome. Hum Mol Genet 24(22) 6390-402(2015) 26319231 |
Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A. Simultaneous detection of multiple transgenes for genetically-modified mouse strains. Exp Anim 58(4) 437-42(2009) 19654444 |