Strain Data Sheet

RBRC01345

Strain Information

Image
BRC No.RBRC01345
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129P2-Emx1<tm1.1(cre)Ito>/ItoRbrc
Former Common nameEmx1-Cre KI Δneo
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
AppearanceBlack [a/a B/B C/C]
Strain development理化学研究所脳科学総合研究センター・岩里琢治、糸原重美。E14 ES細胞を用いて作出。C57BL/6背景。
Strain descriptionEmx1-creノックインマウス。Cre遺伝子とPGK-neoカセットがＥｍｘ1遺伝子の翻訳開始コドンATGの前に挿入されているマウス (neo型) (RBRC00808) とPGK-neoカセットを除去したマウス (delta neo型) (RBRC01345) 。大脳皮質、海馬、嗅球の興奮性神経細胞特異的にCre組換え酵素を発現する。delta neo型マウス (RBRC01345) では、neo型で観察されたectopic expressionが消失しており、内在性遺伝子の発現特性をより反映している。ホモマウスは生存性、繁殖性ともにあり。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesGenesis, 38, 130-138 (2004). 15048810
Nature, 406, 726-731 (2000). 10963597

Health Report

Examination Date / Room / Rack2024/01/09Room:3-ARack:L
2023/10/10Room:3-ARack:L
2023/07/10Room:3-ARack:L
2023/04/10Room:3-ARack:L
2023/01/10Room:3-ARack:L
2022/10/11Room:3-ARack:L
2022/07/11Room:3-ARack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Emx1empty spiracles homolog 1 (Drosophila)6Emx1<tm1.1(cre)Ito>targeted mutation 1.1, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase6cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)6nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6

Ordering Information

供与核酸Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) site, mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Neurobiology Research
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Genesis, 38, 130-138 (2004).
Depositor糸原　重美（独立行政法人理化学研究所）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを3~6ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month June 2006

BRC mice in Publications

Sato T, Ishikawa M, Mochizuki M, Ohta M, Ohkura M, Nakai J, Takamatsu N, Yoshioka K.
JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration.
Cell Death Differ 22(8) 1260-74(2015) 25571974

Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A.
Simultaneous detection of multiple transgenes for genetically-modified mouse strains.
Exp Anim 58(4) 437-42(2009) 19654444

Sugimoto J, Tanaka M, Sugiyama K, Ito Y, Aizawa H, Soma M, Shimizu T, Mitani A, Tanaka K.
Region-specific deletions of the glutamate transporter GLT1 differentially affect seizure activity and neurodegeneration in mice.
Glia 66(4) 777-788(2018) 29214672

Takagi T, Nishizaki Y, Matsui F, Wakamatsu N, Higashi Y.
De novo inbred heterozygous Zeb2/Sip1 mutant mice uniquely generated by germ-line conditional knockout exhibit craniofacial, callosal and behavioral defects associated with Mowat-Wilson syndrome.
Hum Mol Genet 24(22) 6390-402(2015) 26319231

Nagashima S, Ito N, Kobayashi R, Shiiba I, Shimura H, Fukuda T, Hagihara H, Miyakawa T, Inatome R, Yanagi S.
Forebrain-specific deficiency of the GTPase CRAG/Centaurin-γ3 leads to immature dentate gyri and hyperactivity in mice.
J Biol Chem 296 100620(2021) 33811862

Mizusawa A, Watanabe A, Yamada M, Kamei R, Shimomura Y, Kitaura Y.
BDK Deficiency in Cerebral Cortex Neurons Causes Neurological Abnormalities and Affects Endurance Capacity.
Nutrients 12(8) (2020) 32751134