Strain Information | |
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Image | |
BRC No. | RBRC01345 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.129P2-Emx1<tm1.1(cre)Ito>/ItoRbrc |
Former Common name | Emx1-Cre KI delta neo |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C57BL/6JJcl |
Appearance | Black [a/a B/B C/C] |
Strain development | Developed by Takuji Iwasato and Shigeyoshi Itohara, RIKEN Brain Science Institute. E14 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6J. |
Strain description | Emx1-cre knock-in mice. neo type (inserted a cre recombinase gene and a frt flanked PGK-neo cassette into Emx1 gene)(RBRC00808), delta neo type (removed the pgk- neo cassette by FLP mediated recombination)(RBRC01345). The expression of cre recombinase was observed in dorsal telencephalon, neocortex, hippocampus, and olfactory bulb. In the delta neo type (RBRC01345), no ectopic cre expression was detected. Homozygous mice are viable and fertile. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Genesis, 38, 130-138 (2004). 15048810 Nature, 406, 726-731 (2000). 10963597 |
Health Report | |
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Examination Date / Room / Rack | 2024/01/09Room:3-ARack:L 2023/10/10Room:3-ARack:L 2023/07/10Room:3-ARack:L 2023/04/10Room:3-ARack:L 2023/01/10Room:3-ARack:L 2022/10/11Room:3-ARack:L 2022/07/11Room:3-ARack:L 2022/04/11Room:3-ARack:L |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Emx1empty spiracles homolog 1 (Drosophila)6Emx1<tm1.1(cre)Ito>targeted mutation 1.1, Shigeyoshi Itohara Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase6cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)6nls Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA6 |
Ordering Information | |
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Donor DNA | Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) site, mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) genomic DNA |
Research application | Cell Biology Research Cre/loxP system FLP/frt system Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 38, 130-138 (2004). |
Depositor | Shigeyoshi Itohara (RIKEN) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Mouse of the Month June 2006 Genotyping protocol -PCR- Necessary documents for ordering: |
BRC mice in Publications |
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Sato T, Ishikawa M, Mochizuki M, Ohta M, Ohkura M, Nakai J, Takamatsu N, Yoshioka K. JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration. Cell Death Differ 22(8) 1260-74(2015) 25571974 Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A. Simultaneous detection of multiple transgenes for genetically-modified mouse strains. Exp Anim 58(4) 437-42(2009) 19654444 Sugimoto J, Tanaka M, Sugiyama K, Ito Y, Aizawa H, Soma M, Shimizu T, Mitani A, Tanaka K. Region-specific deletions of the glutamate transporter GLT1 differentially affect seizure activity and neurodegeneration in mice. Glia 66(4) 777-788(2018) 29214672 Takagi T, Nishizaki Y, Matsui F, Wakamatsu N, Higashi Y. De novo inbred heterozygous Zeb2/Sip1 mutant mice uniquely generated by germ-line conditional knockout exhibit craniofacial, callosal and behavioral defects associated with Mowat-Wilson syndrome. Hum Mol Genet 24(22) 6390-402(2015) 26319231 Nagashima S, Ito N, Kobayashi R, Shiiba I, Shimura H, Fukuda T, Hagihara H, Miyakawa T, Inatome R, Yanagi S. Forebrain-specific deficiency of the GTPase CRAG/Centaurin-γ3 leads to immature dentate gyri and hyperactivity in mice. J Biol Chem 296 100620(2021) 33811862 Mizusawa A, Watanabe A, Yamada M, Kamei R, Shimomura Y, Kitaura Y. BDK Deficiency in Cerebral Cortex Neurons Causes Neurological Abnormalities and Affects Endurance Capacity. Nutrients 12(8) (2020) 32751134 |