Strain Data Sheet
RBRC01268
Strain Information | |
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| Image | |
| BRC No. | RBRC01268 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129-Numb<tm1Zili> Numbl<tm1Zili>/SiecRbrc |
| Former Common name | Numb [loxP/loxP]; Numblike [loxP/loxP] |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Targeting of Numb：1997, Olav Zilian, ISRECTargeting of Numblike：2001,Olav Zilian, ISREC |
| Strain description | Compound mice conditionally targeted for Numb and Numblike (loxP; for nomenclature of the alleles: lox) ; the selection marker in Numb (loxPneoRloxP) and Numblike (FRTneoRFRT) was deleted by Cre and Flp recombinase, respectively. The mice ready for shipment to RIKEN BRC carry at this point the conditional alleles over a non-targeted, wild-type allele [lox/+]; absence of the transgene FLPe should be reconfirmed in their progeny.Purpose of these conditional alleles: functional analysis of the Numb and Numblike proteins in mice by their conditional ablaion through expression of Cre recombinase. |
| Colony maintenance | Efficient breeding is expected for these compound mice once homozygous for each conditionally targeted allele (Numb[lox / lox]; Numblike[lox / lox]) as they were efficient breeders when the compound homozygotes still carried FRTneoRFRT in both Numblike lox alleles. |
| References | Curr. Biol., 11, 494-501 (2001). 11412999 J. Immunol., 178(11):6746-6751 (2007). 17513721 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP12loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP12loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt |
Ordering Information | |
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| 供与核酸 | phage P1 loxP, mouse Numb genomic DNA, mouse Numblike genomic DNA, yeast FRT (flipase recombination target) site |
| Research application | Cell Biology Research Cre/loxP system |
| 提供条件 | 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。 研究成果の公表にあたって寄託者の指定する文献を引用する。Any publication by the DEPOSITOR describing the BIOLOGICAL RESOURCE for the first time. (For targeted Numb: Olav Zillan et al. Current Biology. 11(7): 494-501; for targeted Numblike : the first publication describing this allele by the DEPOSITOR). This term is to be respected in particular when the first 2 years after deposition of the BIOLOGICAL RESOURCE are passed. 研究成果の公表にあたって謝辞の表明を必要とする。This term is to be respected in particular when the material was requested after the first 2 years upon deposition of the BIOLOGICAL RESOURCE. 1) The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, the RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE in research projects initiated during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. 2) Further, the RECIPIENT must inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR. 3) Furthermore, prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. |
| Depositor | Olav Zilian（Institut Suisse de Recherche Experimentale sur le Cancer (ISREC)） |
| Strain Status |  凍結胚 凍結精子 |
| Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |