Strain Data Sheet

RBRC01252

Strain Information

Image
BRC No.RBRC01252
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(EEF1A1-FLP)66Mim/MmshRbrc
Former Common nameFLP66, FLP66 mouse
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain development開発年:1997年。開発者:竹内倫徳先生、三品昌美先生。開発機関:東京大学大学院医学系研究科薬理学分子神経生物教室C57BL/6N(チャールズリバー)由来。世代数不明。
Strain descriptionヒトペプチド伸長因子サブユニット(EF1-α)プロモーターにより酵母由来組換え酵素Flpを発現するマウス。
Colony maintenanceホモ同士の交配により系統維持。繁殖効率:A。
References
Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting.
Takeuchi T, Nomura T, Tsujita M, Suzuki M, Fuse T, Mori H, Mishina M
Biochem. Biophys. Res. Commun., 293, 953-957 (2002). 12051751

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLP yeast flippase recombinaseUNFLP
MGI:2651460
transgene insertion 66, Masayoshi Mishinahuman elongation factor 1 alpha (EF-1 alpha) promoter
nls SV40 large T antigen nuclear localization signal (NLS)UNnls
poly A human granulocyte colony-stimulating factor gene polyAdenylation signal sequenceUNpoly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
  • Detailed phenotype data

    Ordering Information

    供与核酸human EF1-alpha promoter, SV40 large T antigen nuclear localization signal, yeast flp recombinase, human granulocyte colony-stimulating factor gene poly adenylation signal sequence
    Research applicationCell Biology Research
    FLP/frt system
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Biochem. Biophys. Res. Commun., 293, 953-957 (2002).研究成果の公表にあたって謝辞の表明を必要とする。
    Depositor三品 昌美(国立大学法人東京大学)
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ichise T, Yoshida N, Ichise H.
    H-, N- and Kras cooperatively regulate lymphatic vessel growth by modulating VEGFR3 expression in lymphatic endothelial cells in mice.
    Development 137(6) 1003-13(2010) 20179099
    Fujii T, Ueda T, Nagata S, Fukunaga R.
    Essential role of p400/mDomino chromatin-remodeling ATPase in bone marrow hematopoiesis and cell-cycle progression.
    J Biol Chem 285(39) 30214-23(2010) 20610385
    Ichise H, Ichise T, Ohtani O, Yoshida N.
    Phospholipase Cgamma2 is necessary for separation of blood and lymphatic vasculature in mice.
    Development 136(2) 191-5(2009) 19056831
    Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A.
    Simultaneous detection of multiple transgenes for genetically-modified mouse strains.
    Exp Anim 58(4) 437-42(2009) 19654444