Strain Data Sheet
RBRC01252
Strain Information | |
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| Image | |
| BRC No. | RBRC01252 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6-Tg(EEF1A1-FLP)66Mim/MmshRbrc |
| Former Common name | FLP66, FLP66 mouse |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6NCrlCrlj |
| Appearance | black [a/a B/B C/C] |
| Strain development | 開発年:1997年。開発者:竹内倫徳先生、三品昌美先生。開発機関:東京大学大学院医学系研究科薬理学分子神経生物教室C57BL/6N(チャールズリバー)由来。世代数不明。 |
| Strain description | ヒトペプチド伸長因子サブユニット(EF1-α)プロモーターにより酵母由来組換え酵素Flpを発現するマウス。 |
| Colony maintenance | ホモ同士の交配により系統維持。繁殖効率:A。 |
| References | Biochem. Biophys. Res. Commun., 293, 953-957 (2002). 12051751 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLPyeast flippase recombinaseUNFLPtransgene insertion 66, Masayoshi Mishinahuman elongation factor 1 alpha (EF-1 alpha) promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)UNnls Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly Ahuman granulocyte colony-stimulating factor gene polyAdenylation signal sequenceUNpoly A |
Ordering Information | |
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| 供与核酸 | human EF1-alpha promoter, SV40 large T antigen nuclear localization signal, yeast flp recombinase, human granulocyte colony-stimulating factor gene poly adenylation signal sequence |
| Research application | Cell Biology Research FLP/frt system |
| 提供条件 | 条件を付加する。 研究成果の公表にあたって寄託者の指定する文献を引用する。Biochem. Biophys. Res. Commun., 293, 953-957 (2002). 研究成果の公表にあたって謝辞の表明を必要とする。 |
| Depositor | 三品 昌美（国立大学法人東京大学） |
| Strain Status |  凍結胚 凍結精子 |
| Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Ichise T, Yoshida N, Ichise H. H-, N- and Kras cooperatively regulate lymphatic vessel growth by modulating VEGFR3 expression in lymphatic endothelial cells in mice. Development 137(6) 1003-13(2010) 20179099 Ichise H, Ichise T, Ohtani O, Yoshida N. Phospholipase Cgamma2 is necessary for separation of blood and lymphatic vasculature in mice. Development 136(2) 191-5(2009) 19056831 Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A. Simultaneous detection of multiple transgenes for genetically-modified mouse strains. Exp Anim 58(4) 437-42(2009) 19654444 Fujii T, Ueda T, Nagata S, Fukunaga R. Essential role of p400/mDomino chromatin-remodeling ATPase in bone marrow hematopoiesis and cell-cycle progression. J Biol Chem 285(39) 30214-23(2010) 20610385 |