Strain Data Sheet



BRC No.RBRC01252
SpeciesMus musculus
Strain nameC57BL/6-Tg(EEF1A1-FLP)66Mim/MmshRbrc
Former Common nameFLP66, FLP66 mouse
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Tomonori Takeuchi and Masayoshi Mishina, Graduate School of Medicine, University of Tokyo in 1997. FLP recombinase rexpression vector pEF-NFLP was injected into the pronuclei of C57BL/6NCrj fertilized eggs. Three transgenic mouse lines (FLP22, FLP39, and FLP66) were produced.
Strain descriptionThese transgenic mice express a FLP recombinase gene under the control of the human polypeptide chian elongation factor 1 alpha (EF-1alpha) gene promoter. This can be used to make an inducible knock-out by corssing with the other genetically-engineeered mice that have a pair of FLP recombinase target (FRT) sequence flanking the genomic region of interest. This strain is useful to remove the drug-resistance marker gene from mice manipulated for the conditional gene targeting using the Cre/loxP system.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6NCrlCrlj]. Tg homozygous and hemizygous mice are viable and fertile.
ReferencesBiochem. Biophys. Res. Commun., 293, 953-957 (2002). 12051751

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLPyeast flippase recombinaseUNFLPtransgene insertion 66, Masayoshi Mishinahuman elongation factor 1 alpha (EF-1 alpha) promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly Ahuman granulocyte colony-stimulating factor gene polyAdenylation signal sequenceUNpoly A


Donor DNAhuman EF1-alpha promoter, SV40 large T antigen nuclear localization signal, yeast flp recombinase, human granulocyte colony-stimulating factor gene poly adenylation signal sequence
Research applicationCell Biology Research
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 293, 953-957 (2002).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorMasayoshi Mishina (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Ichise T, Yoshida N, Ichise H.
H-, N- and Kras cooperatively regulate lymphatic vessel growth by modulating VEGFR3 expression in lymphatic endothelial cells in mice.
Development 137(6) 1003-13(2010) 20179099

Ichise H, Ichise T, Ohtani O, Yoshida N.
Phospholipase Cgamma2 is necessary for separation of blood and lymphatic vasculature in mice.
Development 136(2) 191-5(2009) 19056831

Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A.
Simultaneous detection of multiple transgenes for genetically-modified mouse strains.
Exp Anim 58(4) 437-42(2009) 19654444

Fujii T, Ueda T, Nagata S, Fukunaga R.
Essential role of p400/mDomino chromatin-remodeling ATPase in bone marrow hematopoiesis and cell-cycle progression.
J Biol Chem 285(39) 30214-23(2010) 20610385