Strain Data Sheet

RBRC01200

Strain Information

Image
BRC No.RBRC01200
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameNZW.C3-Tg(Mt1-Il5)6Ato
Former Common nameNZW.IL-5
H-2 Haplotype
ES Cell line
Background strainNZW
Appearancealbino [b/b c/c p/p]
Strain developmentGenerated by Kiyoshi Takatsu, Kumamoto University Medical School. The transgene was injected into the pronuclei of C3H fertilized eggs. C57BL/6 (RBRC01908) and BALB/c (RBRC05910) background (Developed by Institute of Medical Science, University of Tokyo), NZW background (RBRC01200) (Developed by Juntendo University School of Medicine).
Strain descriptionMt1-Il5 transgenic mice. These transgenic mice express mouse IL-5 under the control of the Mt1 promoter.
Colony maintenanceCarrier x Noncarrier [or Crossing to NZW/NSlc] Tg mice show no obvious abnormality.
References
Transgenic mice expressing a B cell growth and differentiation factor gene (interleukin 5) develop eosinophilia and autoantibody production.
Tominaga A, Takaki S, Koyama N, Katoh S, Matsumoto R, Migita M, Hitoshi Y, Hosoya Y, Yamauchi S, Kanai Y, et a l
J. Exp. Med., 173, 429-437 (1991). 1988543

Transgene-mediated hyper-expression of IL-5 inhibits autoimmune disease but increases the risk of B cell chronic lymphocytic leukemia in a model of murine lupus.
Wen X, Zhang D, Kikuchi Y, Jiang Y, Nakamura K, Xiu Y, Tsurui H, Takahashi K, Abe M, Ohtsuji M, Nishimura H, Takatsu K, Shirai T, Hirose S
Eur. J. immunol., 34, 2740-2749 (2004). 15368290

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2rabbit beta-globin splice donorUNHBB2
HBB2rabbit beta-globin splice acceptorUNHBB2
Il5interleukin 5UNIl5mouse metallothionein promoter, rabbit beta-globin

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • enlarged spleen(MP:0000691)

  • increased B-1 B cell number(MP:0004977)

  • increased IgA level(MP:0002495)

  • increased IgM level(MP:0002494)

  • increased circulating interleukin-5 level(MP:0008626)
  • more 3 phenotypes
  • increased eosinophil cell number(MP:0005011)

  • increased leukocyte cell number(MP:0000218)

  • increased splenocyte number(MP:0009338)
  • Detailed phenotype data

    Ordering Information

    Donor DNAmouse metallothionein promoter, rabbit beta-globin SD, rabbit beta-globin SA, mouse IL5 cDNA, rabbit beta-globin polyA, SV40 polyadenylation site
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur. J. Immunol., 34, 2740-2749 (2004).
    DepositorSachiko Hirose (Juntendo University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data