Strain Data Sheet

RBRC01195

Strain Information

Image
BRC No.RBRC01195
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S6-Grin2a<tm1Nak>/NakRbrc
Former Common nameNR2A KO, C57BL/6J-Grin2a tm
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C)]
Strain developmentC57BL/6Jに13世代バッククロスしている
Strain descriptionB6.129S6-Grin2a<tm1Nak>. The Grin2a gene is one of the glutamate receptor channel subunit families forming NMDA receptor channel, which is important for synaptic plasticity. Homozygous mutant mice are viable and fertile.
Colony maintenance特記事項なし繁殖効率：A
References
Motor discoordination results from combined gene disruption of the NMDA receptor NR2A and NR2C subunits, but not from single disruption of the NR2A or NR2C subunit.
H Kadotani, T Hirano, M Masugi, K Nakamura, K Nakao, M Katsuki, S Nakanishi
J. Neurosci., 16, 7859-7867 (1996). 8987814

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grin2a
MGI:95820		glutamate receptor, ionotropic, NMDA2A (epsilon 1)16Grin2a<tm1Nak>
MGI:1928506		targeted mutation 1, Shigetada Nakanishi
  • Landau-Kleffner syndrome(MedGEN)
    • neo neomycin resistance gene (E. coli)16 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal excitatory postsynaptic currents (MP:0002910)

  • abnormal miniature excitatory postsynaptic currents (MP:0004753)

  • decreased vertical activity (MP:0002757)
    • Detailed phenotype data

    Ordering Information

    供与核酸herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse NMDA receptor NR2A subunit genomic DNA
    Research applicationNeurobiology Research
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。J. Neurosci., 16, 7859-7867 (1996). 研究成果の公表にあたって謝辞の表明を必要とする。
    Depositor中西　重忠（国立大学法人京都大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Lu Y, Mu L, Elstrott J, Fu C, Sun C, Su T, Ma X, Yan J, Jiang H, Hanson JE, Geng Y, Chen Y.
    Differential depletion of GluN2A induces heterogeneous schizophrenia-related phenotypes in mice.
    EBioMedicine 102 105045(2024) 38471394
    Su T, Lu Y, Fu C, Geng Y, Chen Y.
    GluN2A mediates ketamine-induced rapid antidepressant-like responses.
    Nat Neurosci (2023) 37709995
    Gao X, Qiao Y, Jia B, Jing X, Cheng B, Wen L, Tan Q, Zhou Y, Zhu B, Qiao H.
    NMDA Receptor-Dependent Synaptic Activity in Dorsal Motor Nucleus of Vagus Mediates the Enhancement of Gastric Motility by Stimulating ST36.
    Evid Based Complement Alternat Med 2012 438460(2012) 23118791