Strain Data Sheet

RBRC01191

Strain Information

Image
BRC No.RBRC01191
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Meis1<tm1Ngc>/Rbrc
Former Common nameMeis1 Knockout mice
H-2 Haplotype
ES Cell lineJR2448 [129S3/SvImJ]
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain development開発年：1997年開発者：Masami Fujita & Neal Copeland機関名：米国 National Cancer Institute129Sv由来のES細胞を用いた　C57BL/6J系で維持　8世代
Strain descriptionMeis1のTargeted mutation mouse。Meis1 koのヘテロマウスはB6 backgroundの場合かなりのものがdpc 14.5-16.5で出血のため胎内死亡となります。129 backgroundの場合はこの現象は見られないようです。
Colony maintenancelitter size　やや小さいホモで不妊／死亡
ReferencesEMBO J., 23, 450-459 (2004). 14713950

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Meis1myeloid ecotropic viral integration site 111Meis1<tm1Ngc>targeted mutation 1, Neal G Copeland

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)11GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸adenovirus major late transcript splice acceptor (SA) sequence, E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine growth hormone poly A, mouse Meis1 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Sensorineural Research
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。EMBO J., 23, 450-459 (2004).
Depositor中村　卓郎（(財)癌研究会癌研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data