Strain Data Sheet

RBRC01191

Strain Information

Image
BRC No.RBRC01191
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Meis1<tm1Ngc>/Rbrc
Former Common nameMeis1 Knockout mice
H-2 Haplotype
ES Cell lineJR2448 [129S3/SvImJ]
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Masami Fujita and Neal Copeland, National Cancer Institute in 1997. Donor strain is 129S3/SvlmJ via JR2448 ES cell line. This strain was maintained in C57BL/6J and 129S3/SvlmJ mixed background.
Strain descriptionB6;129S3-Meis1<tm1Ngc>. Meis1 deficient mice exhibit abnormal retina, vasculature, hematopoietic system defects. Homozygous mutant mice die by embryonic day 14.5.
Colony maintenanceLitter size is a little small. Embryonic lethality (Homozygous). Genotyping by PCR.
References
Hematopoietic, angiogenic and eye defects in Meis1 mutant animals.
Hisa T, Spence S E, Rachel R A, Fujita M, Nakamura T, Ward J M, Devor-Henneman D E, Saiki Y, Kutsuna H, Tessarollo L, Jenkins N A, Copeland N G
EMBO J., 23, 450-459 (2004). 14713950

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GHGrowth hormone polyA (Bovine)11GH
Meis1Meis homeobox 111Meis1targeted mutation 1, Neal G Copeland
lacZbeta-galactosidase (E. coli)11
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal angiogenesis(MP:0000260)

  • abnormal brain morphology(MP:0002152)

  • abnormal capillary branching pattern(MP:0020327)

  • abnormal capillary morphology(MP:0003658)

  • abnormal heart morphology(MP:0000266)
  • more 11 phenotypes
  • abnormal kidney morphology(MP:0002135)

  • abnormal liver morphology(MP:0000598)

  • abnormal lung morphology(MP:0001175)

  • abnormal retina morphology(MP:0001325)

  • abnormal vascular branching morphogenesis(MP:0003227)

  • absent megakaryocytes(MP:0008253)

  • decreased common myeloid progenitor cell number(MP:0008813)

  • hemorrhage(MP:0001914)

  • lethality throughout fetal growth and development, complete penetrance(MP:0011099)

  • limbs/digits/tail phenotype(MP:0005371)

  • small lens(MP:0001306)
  • Detailed phenotype data

    Ordering Information

    Donor DNAadenovirus major late transcript splice acceptor (SA) sequence, E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine growth hormone poly A, mouse Meis1 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Sensorineural Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 23, 450-459 (2004).
    DepositorTakuro Nakamura (Japanese Foundation for Cancer Research)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data