Strain Information | |
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Image | |
BRC No. | RBRC01191 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Meis1<tm1Ngc>/Rbrc |
Former Common name | Meis1 Knockout mice |
H-2 Haplotype | |
ES Cell line | JR2448 [129S3/SvImJ] |
Background strain | C57BL/6J |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Drs. Masami Fujita and Neal Copeland, National Cancer Institute in 1997. Donor strain is 129S3/SvlmJ via JR2448 ES cell line. This strain was maintained in C57BL/6J and 129S3/SvlmJ mixed background. |
Strain description | B6;129S3-Meis1<tm1Ngc>. Meis1 deficient mice exhibit abnormal retina, vasculature, hematopoietic system defects. Homozygous mutant mice die by embryonic day 14.5. |
Colony maintenance | Litter size is a little small. Embryonic lethality (Homozygous). Genotyping by PCR. |
References | Hematopoietic, angiogenic and eye defects in Meis1 mutant animals. Hisa T, Spence S E, Rachel R A, Fujita M, Nakamura T, Ward J M, Devor-Henneman D E, Saiki Y, Kutsuna H, Tessarollo L, Jenkins N A, Copeland N G EMBO J., 23, 450-459 (2004). 14713950 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
GH | Growth hormone polyA (Bovine) | 11 | GH | ||||
Meis1 | Meis homeobox 1 | 11 | Meis1 | targeted mutation 1, Neal G Copeland | |||
lacZ | beta-galactosidase (E. coli) | 11 | |||||
neo | neomycin resistance gene (E. coli) | 11 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 11 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | adenovirus major late transcript splice acceptor (SA) sequence, E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine growth hormone poly A, mouse Meis1 genomic DNA |
Research application | Fluorescent Proteins/lacZ System Sensorineural Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 23, 450-459 (2004). |
Depositor | Takuro Nakamura (Japanese Foundation for Cancer Research) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |