Strain Data Sheet

RBRC01190

Strain Information

Image
BRC No.RBRC01190
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Bcl11a<tm1Ngc>/Rbrc
Former Common nameEvi9/BclIIa Knockout mice
H-2 Haplotype
ES Cell lineCJ7 [129S1/SvImJ]
Background strain
Appearancewhite-bellied agouti [A<w>/A<w> B/B C/C]
Strain developmentDeveloped by Drs. Pentao Liu and Neal G Copeland at NIH in 1999. A PGK-neo cassette replaced was transfered into CJ7 ES cells to replace exon 1 of Bcl11a gene.
Strain descriptionDeveloped by Drs. Pentao Liu and Neal G Copeland at NIH in 1999. A PGK-neo cassette replaced was transfered into CJ7 ES cells to replace exon 1 of Bcl11a gene.
Colony maintenanceSibling Mating (Heterozygote x Wild-type, Heterozygote)Homozygous mutant mice die within 3-4hrs after birth.
References
Bcl11a is essential for normal lymphoid development.
Liu P, Keller J R, Ortiz M, Tessarollo L, Rachel R A, Nakamura T, Jenkins N A, Copeland N G
Nat. Immunol., 4, 525-532 (2003). 12717432

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bcl11a
MGI:106190		BCL11 transcription factor A11Bcl11a<tm1Ngc>
MGI:2663941		targeted mutation 1, Neal G Copeland
  • Dias-Logan syndrome(MedGEN)
    • GH Growth hormone polyA (Bovine)11GH
    SA adenovirus major late transcript splice acceptor (SA) sequence11SA
    lacZ beta-galactosidase (E. coli)11
    neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal T cell differentiation (MP:0002145)

  • abnormal common lymphocyte progenitor cell morphology (MP:0008249)

  • abnormal dendritic cell differentiation (MP:0008115)

  • abnormal immunoglobulin heavy chain V(D)J recombination (MP:0008760)

  • absent B cells (MP:0008071)
    • more 13 phenotypes
  • absent CD4-positive, alpha-beta T cells (MP:0003790)

  • absent lymphocyte (MP:0000726)

  • decreased DN2 thymocyte number (MP:0010132)

  • decreased T cell number (MP:0005018)

  • decreased hematopoietic stem cell number (MP:0004810)

  • decreased lymphocyte cell number (MP:0005016)

  • decreased plasmacytoid dendritic cell number (MP:0008124)

  • increased CD8-positive, alpha-beta T cell number (MP:0008078)

  • increased double-negative T cell number (MP:0005090)

  • increased gamma-delta T cell number (MP:0008346)

  • neonatal lethality, complete penetrance (MP:0011087)

  • small thymus (MP:0000706)

  • thymus hypoplasia (MP:0001823)
    • Detailed phenotype data

    Ordering Information

    Donor DNAadenovirus major late transcript splice acceptor (SA) sequence, E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine growth hormone poly A, mouse Bcl11a genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Immunology and Inflammation Research
    covid19_progression
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Immunol., 4, 525-532 (2003).
    DepositorTakuro Nakamura (Japanese Foundation for Cancer Research)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Simbula M, Manchinu MF, Mingoia M, Pala M, Asunis I, Caria CA, Perseu L, Shah M, Crossley M, Moi P, Ristaldi MS.
    miR-365-3p mediates BCL11A and SOX6 erythroid-specific coregulation: A new player in HbF activation.
    Mol Ther Nucleic Acids 34 102025(2023) 37744176