Strain Data Sheet

RBRC01153

Strain Information

Image
BRC No.RBRC01153
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Six3-cre)22Frty
Former Common nameTgN(Six3 Cre)22Frt; Six3Cre#22
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yasuhide Furuta, University of Texas, M.D. Anderson Cancer Center. The Six3-cre transgene fragment was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were crossed to C57BL/6 (N5 times).
Strain descriptionTransgenic mice expressing Cre recombinase in a central region of the developing retina and widely in the ventral forebrain. Useful for tissue-specific gene activation/inactivation approaches. In particular, consequences of gene activation/inactivation in Cre-expressing cells within the retina can be assessed in direct comparisons to the surrounding non-Cre-expressing cells in an animal. Line 69 (RBRC01154), Line 22 (RBRC01153).
Colony maintenanceCarrier x Carrier
References
Retina- and ventral forebrain-specific Cre recombinase activity in transgenic mice.
Furuta Y, Lagutin O, Hogan B L, Oliver G C
Genesis, 26, 130-132 (2000). 10686607

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GHGrowth hormone polyA (Bovine)UNGH
  • holoprosencephaly 2(DisGeNET, MedGEN)

  • schizencephaly(MedGEN)
    • crePhage P1 Cre recombinaseUNcremouse Six3 promoter
  • holoprosencephaly 2(DisGeNET, MedGEN)

  • schizencephaly(MedGEN)
    • nlsSV40 large T antigen nuclear localization signal (NLS)UNnls
  • holoprosencephaly 2(DisGeNET, MedGEN)

  • schizencephaly(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse six3 genomic DNA, SV40-T-derived nuclear localization signal (NLS), phage P1 Cre recombinase, bovine Growth Hormone poly-A signal
    Research applicationCell Biology Research
    Cre/loxP system
    Genetics Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 26, 130-132 (2000).
    DepositorYasuhide Furuta (The University of Texas M. D. Anderson Cancer Center)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month June 2006

    BRC mice in Publications

    No Data