Strain Data Sheet

RBRC01151

Strain Information

Image
BRC No.RBRC01151
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S7-Hey1<tm1Kkb>/YsaRbrc
Former Common namehesr1, hairy and enhancer of split-related 1
H-2 Haplotype
ES Cell lineAB1 [129S7/SvEvBrd-Hprt<+>]
Background strainC57BL/6NJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. H. Kokubo and R.L. Johnson at University of Texas M.D. Anderson Cancer Center in 1999. A loxP-flanked neo cassette was transferred into AB1 ES cells to replace the bHLH region, from exon 1 to exon 4. C57BL/6 and 129 mixed background.
Strain descriptionB6;129S7-Hey1<tm1Kkb>. Hey1 gene is a member of the hairy and enhancer of split related family of bHLH transcription factors, play a important roles in mediating Notch signaling in the developing cardiac and vascular systems.
Colony maintenanceSibling MatingHomozygous mutant mice are viable and fertile.
References
Mouse hesr1 and hesr2 genes are redundantly required to mediate Notch signaling in the developing cardiovascular system.
Kokubo H, Miyagawa-Tomita S, Nakazawa M, Saga Y, Johnson R L
Dev Biol., 278,　301-309 (2005）. 15680351

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Hey1hairy/enhancer-of-split related with YRPW motif 13Hey1targeted mutation 1, Hiroki Kokubo
IRESinternal ribosomal entry site (EMCV)3
lacZbeta-galactosidase (E. coli)3
loxPphage P1 loxP3loxP
loxPphage P1 loxP3loxP
neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • muscle phenotype(MP:0005369)

  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse hairy and enhancer of split-related 1 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Harada Y, Yamada M, Imayoshi I, Kageyama R, Suzuki Y, Kuniya T, Furutachi S, Kawaguchi D, Gotoh Y.
    Cell cycle arrest determines adult neural stem cell ontogeny by an embryonic Notch-nonoscillatory Hey1 module.
    Nat Commun 12(1) 6562(2021) 34772946