Strain Data Sheet

RBRC01145

Strain Information

Image
BRC No.RBRC01145
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Mesp1<tm2(cre)Ysa>/YsaRbrc
Former Common nameMesp1-cre
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainICR MCH
AppearanceAgouti or black [A/? or a/a B/B Mesp1-Cre c/+ C]
albino [A/? or a/a B/B + c/+ C]
Strain development開発年：1998年開発者：相賀裕美子先生機関名：国立医薬品食品衛生研究所
Strain descriptionMesp1 遺伝子座 cre recombinase が発現。
Colony maintenance第7番染色体にのっており、tyrosinase gene と近いので、アルビノと交配すると、targeted allele を持つものは、茶か黒になる (ほとんどアグーチ）。交配に供したICRの購入元：クレア（MCH)
References
MesP1 is expressed in the heart precursor cells and required for the formation of a single heart tube.
Saga Y, Miyagawa-Tomita S, Takagi A, Kitajima S, Miyazaki J i, Inoue T
Development, 126, 3437-3447 (1999). 10393122

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mesp1
MGI:107785		mesoderm posterior 17Mesp1<tm2(cre)Ysa>
MGI:2176467		targeted mutation 2 (cre), Yumiko Saga
cre Phage P1 Cre recombinase7cre Mesp1
neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    供与核酸phage P1 Cre recombinase, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Mesp1 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Development, 126, 3437-3447 (1999).
    Depositor相賀　裕美子（国立遺伝学研究所）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Feb 2006
    Genetic Background

    BRC mice in Publications

    Tuwatnawanit T, Wessman W, Belisova D, Sumbalova Koledova Z, Tucker AS, Anthwal N.
    FSP1/S100A4-Expressing Stem/Progenitor Cells Are Essential for Temporomandibular Joint Growth and Homeostasis.
    J Dent Res 220345251313795(2025) 39953712
    Funato N, Heliövaara A, Boeckx C.
    A regulatory variant impacting TBX1 expression contributes to basicranial morphology in Homo sapiens.
    Am J Hum Genet (2024) 38608674
    Funato N, Srivastava D, Shibata S, Yanagisawa H.
    TBX1 Regulates Chondrocyte Maturation in the Spheno-occipital Synchondrosis.
    J Dent Res 99(10) 1182-1191(2020) 32442036
    Funato N, Nakamura M, Richardson JA, Srivastava D, Yanagisawa H.
    Loss of Tbx1 induces bone phenotypes similar to cleidocranial dysplasia.
    Hum Mol Genet 24(2) 424-35(2015) 25209980
    Thompson H, Ohazama A, Sharpe PT, Tucker AS.
    The origin of the stapes and relationship to the otic capsule and oval window.
    Dev Dyn 241(9) 1396-404(2012) 22778034
    Rothova M, Thompson H, Lickert H, Tucker AS.
    Lineage tracing of the endoderm during oral development.
    Dev Dyn 241(7) 1183-91(2012) 22581563
    Randall V, McCue K, Roberts C, Kyriakopoulou V, Beddow S, Barrett AN, Vitelli F, Prescott K, Shaw-Smith C, Devriendt K, Bosman E, Steffes G, Steel KP, Simrick S, Basson MA, Illingworth E, Scambler PJ.
    Great vessel development requires biallelic expression of Chd7 and Tbx1 in pharyngeal ectoderm in mice.
    J Clin Invest 119(11) 3301-10(2009) 19855134
    Domínguez-Frutos E, Vendrell V, Alvarez Y, Zelarayan LC, López-Hernández I, Ros M, Schimmang T.
    Tissue-specific requirements for FGF8 during early inner ear development.
    Mech Dev 126(10) 873-81(2009) 19619645
    MacDonald ST, Bamforth SD, Chen CM, Farthing CR, Franklyn A, Broadbent C, Schneider JE, Saga Y, Lewandoski M, Bhattacharya S.
    Epiblastic Cited2 deficiency results in cardiac phenotypic heterogeneity and provides a mechanism for haploinsufficiency.
    Cardiovasc Res 79(3) 448-57(2008) 18440989
    Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM.
    Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
    Cell Stem Cell 3(1) 55-68(2008) 18593559