Strain Data Sheet

RBRC01145

Strain Information

Image
BRC No.RBRC01145
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Mesp1<tm2(cre)Ysa>/YsaRbrc
Former Common nameMesp1-cre
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainICR MCH
AppearanceAgouti or black [A/? or a/a B/B Mesp1-Cre c/+ C]
albino [A/? or a/a B/B + c/+ C]
Strain developmentDeveloped by Yumiko Sga, National Institute of Health Sciences in 1998. A Cre gene and pgk-neo cassette were ligated in frame at a NcoI site located at the translational initiation site of Mesp1 gene. ICR mixed background.
Strain descriptionMesp1-Cre knock-in mice, in which Cre recombinase is expressed under the control of the endogenous promoter-enhancer. By crossing with a mouse strain with a reporter gene like a GFP and LacZ which is interrupted by a floxed CAT gene, it is possible to detect Mesp1-expressing cells. Mesp1 is a transcriptional factor temporarily expressing in endothelium of the blood vessel in a part of lateral plate mesoderm and heart cells, and is a determinant that is important for the formation of heart. This strain is useful for understanding of the mechanisms underlying cardiovascular development. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)]. Homozygous mutant mice are embryonic lethal.
References
MesP1 is expressed in the heart precursor cells and required for the formation of a single heart tube.
Saga Y, Miyagawa-Tomita S, Takagi A, Kitajima S, Miyazaki J i, Inoue T
Development, 126, 3437-3447 (1999). 10393122

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mesp1mesoderm posterior 17Mesp1targeted mutation 2 (cre), Yumiko Saga
crePhage P1 Cre recombinase7creMesp1
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 Cre recombinase, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Mesp1 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 126, 3437-3447 (1999).
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Feb 2006
    Genetic Background

    BRC mice in Publications

    Funato N, Heliövaara A, Boeckx C.
    A regulatory variant impacting TBX1 expression contributes to basicranial morphology in Homo sapiens.
    Am J Hum Genet (2024) 38608674
    MacDonald ST, Bamforth SD, Chen CM, Farthing CR, Franklyn A, Broadbent C, Schneider JE, Saga Y, Lewandoski M, Bhattacharya S.
    Epiblastic Cited2 deficiency results in cardiac phenotypic heterogeneity and provides a mechanism for haploinsufficiency.
    Cardiovasc Res 79(3) 448-57(2008) 18440989
    Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM.
    Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
    Cell Stem Cell 3(1) 55-68(2008) 18593559
    Thompson H, Ohazama A, Sharpe PT, Tucker AS.
    The origin of the stapes and relationship to the otic capsule and oval window.
    Dev Dyn 241(9) 1396-404(2012) 22778034
    Rothova M, Thompson H, Lickert H, Tucker AS.
    Lineage tracing of the endoderm during oral development.
    Dev Dyn 241(7) 1183-91(2012) 22581563
    Funato N, Nakamura M, Richardson JA, Srivastava D, Yanagisawa H.
    Loss of Tbx1 induces bone phenotypes similar to cleidocranial dysplasia.
    Hum Mol Genet 24(2) 424-35(2015) 25209980
    Randall V, McCue K, Roberts C, Kyriakopoulou V, Beddow S, Barrett AN, Vitelli F, Prescott K, Shaw-Smith C, Devriendt K, Bosman E, Steffes G, Steel KP, Simrick S, Basson MA, Illingworth E, Scambler PJ.
    Great vessel development requires biallelic expression of Chd7 and Tbx1 in pharyngeal ectoderm in mice.
    J Clin Invest 119(11) 3301-10(2009) 19855134
    Funato N, Srivastava D, Shibata S, Yanagisawa H.
    TBX1 Regulates Chondrocyte Maturation in the Spheno-occipital Synchondrosis.
    J Dent Res 99(10) 1182-1191(2020) 32442036
    Domínguez-Frutos E, Vendrell V, Alvarez Y, Zelarayan LC, López-Hernández I, Ros M, Schimmang T.
    Tissue-specific requirements for FGF8 during early inner ear development.
    Mech Dev 126(10) 873-81(2009) 19619645