Strain Data Sheet

RBRC01142

Strain Information

Image
BRC No.RBRC01142
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(CAG-cat,-Notch1)1Ysa/YsaRbrc
Former Common nameCAG-CAT-Notch1
H-2 Haplotype
ES Cell line
Background strainJcl:MCH(ICR)
Appearancealbino [c/c]
Strain developmentDeveloped by Dr. Yumiko Saga (2002). The transgene fragment was injected into the pronuclei of B6C3F1 fertilized eggs. This strain was maintained with ICR background.
Strain descriptionNotch1, Notch gene homolog 1 (Drosophila) is imortant in establishing metameric pattern during somitogenesis. This transgenic mouse expresses the active Notch1 gene after mating with a cre transgenic mouse. This strain are useful for elucidation of the Notch-signalling mechanism underlying initial segmentation in the presomitic mesoderm.
Colony maintenanceSibling Mating
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Notch1Notch gene homolog 1 (Drosophila)UNNotch1
  • Adams-Oliver syndrome 5(MedGEN)

  • aortic valve disease 1(MedGEN)
    • catE. coli chloramphenicol acetyltransferase (CAT)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
  • Adams-Oliver syndrome 5(MedGEN)

  • aortic valve disease 1(MedGEN)
    • loxPphage P1 loxPUNloxP
  • Adams-Oliver syndrome 5(MedGEN)

  • aortic valve disease 1(MedGEN)
    • loxPphage P1 loxPUNloxP
  • Adams-Oliver syndrome 5(MedGEN)

  • aortic valve disease 1(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, E. coli chloramphenicol acetyltransferase (CAT) gene, poly A, Notch1 intracellular domain cDNA, poly A
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYumiko Saga (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Saga Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Kratsios P, Catela C, Salimova E, Huth M, Berno V, Rosenthal N, Mourkioti F.
    Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart.
    Circ Res 106(3) 559-72(2010) 20007915