Strain Data Sheet

RBRC01131

Strain Information

Image
BRC No.RBRC01131
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-p35)1Miur/Rbrc
Former Common nameCAG-loxP-p35 Tg mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Hisahara S, Araki T and Miura M at Osaka Univ. Graduate School Med. in 2000. The mice were generated by using C57BL/6 fertilized eggs.
Strain descriptionThis transgenic mouse expresses the baculovirus anti-apoptotic protein p35 in oligodendrocytes through the Cre-loxP system.
Colony maintenanceBoth hemizygous and homozygous transgenic mice can be bred as well as wild-type C57BL/6 mice.
References
Targeted expression of baculovirus p35 caspase inhibitor in oligodendrocytes protects mice against autoimmune-mediated demyelination.
Hisahara S, Araki T, Sugiyama F, Yagami K i, Suzuki M, Abe K, Yamamura K, Miyazaki J, Momoi T, Saruta T, Bernard C C, Okano H, Miura M
EMBO J., 19, 341-348 (2000). 10654933

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
loxPphage P1 loxPUNloxP
loxPphage P1 loxPUNloxP
neoneomycin resistance gene (E. coli)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
p35baculovirus anti-apoptotic protein p35UNp35

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • autoimmune response(MP:0001844)

  • decreased apoptosis(MP:0006043)

  • decreased susceptibility to experimental autoimmune encephalomyelitis(MP:0004800)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), baculovirus anti-apoptotic protein p35, E. coli neo, phage P1 loxP, SV40 poly A
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 19, 341-348 (2000).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The material shall be used for teaching or not-for-profit research purposes only for the research described within this MTA.
    DepositorMasayuki Miura (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data