Strain Data Sheet

RBRC01130

Strain Information

Image
BRC No.RBRC01130
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Ugt8a<tm1Pop>/Rbrc
Former Common nameCeramide galactosyltransferase (CGT)-deficient mice
H-2 Haplotype
ES Cell lineBK4 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Brain Popko, University of North Carolina in 1996.
Strain descriptionCGT-deficient mice fail to make galactolipid galactocerebroside and its sulfated derivative that are normal myelin constitutents. Mutant mice exhibit tremors, ataxia, progressive hindlib paralysis and vaculoe formation in ventral spinal cord due to abnormal myelin sheath.B6.129P2-Ugt8a<tm1Pop>. Ceramide galactosyltransferase, CGT is a key enzyme in the biosynthesis of galactocerebroside, GalC. Homozygous mutant mice for CGT gene show tremor, ataxia progressive hind limb paralysis and extensiv vacuolation of the ventral spinal cord.
Colony maintenancehetero x heterohomozygous mice are infertile.行動の異常がある形態の異常・奇形がある繁殖成績が低いホモで不妊／死亡
ReferencesJ. Lipid Res., 41, 1237-1243 (2000). 10946011
蛋白質 核酸 酵素, 43,2318-2324 (1998). 9883654
Cell, 86, 209-219 (1996). 8706126
J. Biol. Chem., 275, 22623-22626 (2000). 10801776
蛋白質 核酸 酵素, 46, 45-54 (2001). 11193331

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ugt8UDP-glucuronosyltransferase 83Ugt8<tm1Pop>targeted mutation 1, Brian PopkoCgt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3neo; neomycin;

Ordering Information

供与核酸mouse Ceramide galactosyltransferase (CGT) genomic DNA, E. coli neo
Research applicationCell Biology Research
Neurobiology Research
提供条件条件を付加しない。
DepositorBrian Popko（University of Chicago）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data