Strain Data Sheet

RBRC01122

Strain Information

Image
BRC No.RBRC01122
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(REN)8-12Afu/AfuRbrc
Former Common namehRN 8-12
H-2 Haplotype
ES Cell line
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Akiyoshi Fukamizu, University of Tsukuba in 1991. C57BL/6 background.
Strain descriptionHuman renin gene transgenic mice with integration of 10 copies of a 15 kbp human DNA fragment containing the renin gene (hRN 8-12 mice). The renin-angiotensin system (RAS) is one of the determinant factors in regulating blood pressure and sodium absorption. RAS has been shown to accelerate various diseases such as renal failure, atherosclerosis, cardiac hypertrophy, and pulmonary hypertension. The reaction between renin, produced in the kidney, and its substrate angiotensinogen, produced in the liver, is the initial and rate-limiting step in the enzymatic cascade that generates angiotensin I. Angiotensin-converting enzyme in the lung converts angiotensin I to the potent vasoconstrictor angiotensin II (Ang II). The depositor reported that overactivation of the RAS induces hypertension in the F1 generation obtained from breeding between a female carrying the human renin gene (hRN+/+) and a male possessing the human angiotensinogen gene (hAG+/+). Moreover, female hAG+/+ mice when bred with male hRN+/+ mice are hypertensive specifically in late pregnancy, suggesting possible implications for preeclampsia or pregnancy-induced hypertension, a life-threatening disorder for human mothers and fetuses. hRN+/+ and hANG+/+ mice provide the opportunity to understand the role of the RAS in hypertension and other common diseases. hRN8-12 mice (RBRC01122), hAG 2-5 mice (RBRC01123), hRN8-12 x hAG2-5)F1 mice (RBRC02432).
Colony maintenanceCarrier x Carrier (Homozygote x Homozygote) Homozygous mice are viable and fertile.
References
Tissue-specific expression of the human renin gene in transgenic mice.
Fukamizu A, Seo M S, Hatae T, Yokoyama M, Nomura T, Katsuki M, Murakami K
Biochem. Biophys. Res. Commun., 165, 826-832 (1989). 2480785

Health Report

Examination Date / Room / Rack2024/09/09Room:3-2Rack:C
2024/06/10Room:3-2Rack:C
2024/03/11Room:3-2Rack:C
2023/12/11Room:3-2Rack:C
2023/09/11Room:3-2Rack:C
2023/06/13Room:3-2Rack:C
2023/03/13Room:3-2Rack:C

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
REN1renin 1 structural (human)UNREN1

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
  • Detailed phenotype data

    Ordering Information

    Donor DNAhuman renin1 genomic DNA (10 exons, 9 introns and native promoter)
    Research applicationCell Biology Research
    Hematological Research
    covid19_progression
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 165, 826-832 (1989).
    DepositorAkiyoshi Fukamizu (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month May 2012
    Systolic blood pressure

    BRC mice in Publications

    Inaba S, Iwai M, Furuno M, Tomono Y, Kanno H, Senba I, Okayama H, Mogi M, Higaki J, Horiuchi M.
    Continuous activation of renin-angiotensin system impairs cognitive function in renin/angiotensinogen transgenic mice.
    Hypertension 53(2) 356-62(2009) 19047580
    Falcao S, Bisotto S, Michel C, Lacasse AA, Vaillancourt C, Gutkowska J, Lavoie JL.
    Exercise training can attenuate preeclampsia-like features in an animal model.
    J Hypertens 28(12) 2446-53(2010) 20811291
    Ogawa M, Suzuki J, Takayama K, Senbonmatsu T, Hirata Y, Nagai R, Isobe M.
    Impaired post-infarction cardiac remodeling in chronic kidney disease is due to excessive renin release.
    Lab Invest 92(12) 1766-76(2012) 22986786
    Inaba S, Iwai M, Tomono Y, Senba I, Furuno M, Kanno H, Okayama H, Mogi M, Higaki J, Horiuchi M.
    Exaggeration of focal cerebral ischemia in transgenic mice carrying human Renin and human angiotensinogen genes.
    Stroke 40(2) 597-603(2009) 19023100