Strain Data Sheet

RBRC01121

Strain Information

Image
BRC No.RBRC01121
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ren1<tm1Afu>/AfuRbrc
Former Common nameREN-KO (BL/6)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain development開発年：2000年開発者：深水昭吉先生機関名：筑波大学C57BL/6J (日本クレア）　　10世代
Strain description内在のレニン遺伝子を欠損し、低血圧を呈する。
Colony maintenance繁殖効率：BInbred Strain をback groundとした場合には特にhomo化した場合に繁殖が困難。本系統を繁殖させる場合は、hetero欠損マウスを母親に用い、産後３週間ほど産仔に生理食塩水を皮下投与することで、産仔数の７割程度の離乳個体を得ることができる。ホモ化した場合、〜50％死亡する可能性あり。
References
Renin-dependent cardiovascular functions and renin-independent blood-brain barrier functions revealed by renin-deficient mice.
K Yanai, T Saito, Y Kakinuma, Y Kon, K Hirota, K Taniguchi-Yanai, N Nishijo, Y Shigematsu, H Horiguchi, Y Kasuya, F Sugiyama, K i Yagami, K Murakami, A Fukamizu
J. Biol. Chem., 275, 5-8 (2000). 10617578

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ren1
MGI:97898		renin 1 structural1Ren1<tm1Afu>
MGI:2136642		targeted mutation 1, Akiyoshi Fukamizu
  • familial juvenile hyperuricemic nephropathy type 2(MedGEN)

  • renal tubular dysgenesis of genetic origin(MedGEN)
    • lacZ beta-galactosidase (E. coli)1
    neo neomycin resistance gene (E. coli)1

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal blood homeostasis (MP:0009642)

  • abnormal innervation (MP:0002184)

  • abnormal juxtaglomerular apparatus morphology (MP:0002829)

  • abnormal juxtaglomerular cell morphology (MP:0011300)

  • abnormal macula densa morphology (MP:0011299)
    • more 10 phenotypes
  • decreased circulating renin level (MP:0003353)

  • decreased systemic arterial blood pressure (MP:0002843)

  • decreased systemic arterial diastolic blood pressure (MP:0006263)

  • decreased systemic arterial systolic blood pressure (MP:0006264)

  • hydronephrosis (MP:0000519)

  • kidney atrophy (MP:0004970)

  • polydipsia (MP:0001426)

  • polyuria (MP:0001762)

  • postnatal lethality, complete penetrance (MP:0011085)

  • vascular smooth muscle hyperplasia (MP:0005489)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse renin 1 genomic DNA, E. coli neo, E. coli lacZ
    Research applicationCell Biology Research
    Hematological Research
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。J. Biol. Chem., 275, 5-8 (2000).
    Depositor深水　昭吉（国立大学法人筑波大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data