Strain Data Sheet



BRC No.RBRC01121
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ren1<tm1Afu>/AfuRbrc
Former Common nameREN-KO (BL/6)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Strain developmentDeveloped by Dr. Keiji Tanimoto, University of Tsukuba in 2003. C57BL/6 background.
Strain descriptionB6.Cg-Ren1<tm1Afu>. Renin plays an essential role in the control of blood pressure through its specific leavage of angiotensinogen to generate angiotensin I. Homozygous mutant mice exhibit lowered blood pressure, increased urine and drinking volume, and altered renal morphology.
Colony maintenanceSibling Mating (Heterozygote x Heterozygote, Wild-type) Homozygous mutant mice lowered blood pressure and die within a week after birth.
ReferencesJ. Biol. Chem., 275, 5-8 (2000). 10617578

Health Report

Examination Date / Room / Racknull


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ren1renin 1 structural1Ren1<tm1Afu>targeted mutation 1, Akiyoshi FukamizuRen, Ren-1, Ren-A, Ren1d, Rn-1, Rnr

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)1neo; neomycin;


Donor DNAmouse renin 1 genomic DNA, E. coli neo, E. coli lacZ
Research applicationCell Biology Research
Hematological Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 275, 5-8 (2000).
DepositorAkiyoshi Fukamizu (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data