Strain Data Sheet

RBRC01121

Strain Information

Image
BRC No.RBRC01121
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ren1<tm1Afu>/AfuRbrc
Former Common nameREN-KO (BL/6)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Keiji Tanimoto, University of Tsukuba in 2003. C57BL/6 background.
Strain descriptionB6.Cg-Ren1<tm1Afu>. Renin plays an essential role in the control of blood pressure through its specific leavage of angiotensinogen to generate angiotensin I. Homozygous mutant mice exhibit lowered blood pressure, increased urine and drinking volume, and altered renal morphology.
Colony maintenanceSibling Mating (Heterozygote x Heterozygote, Wild-type) Homozygous mutant mice lowered blood pressure and die within a week after birth.
References
Renin-dependent cardiovascular functions and renin-independent blood-brain barrier functions revealed by renin-deficient mice.
Yanai K, Saito T, Kakinuma Y, Kon Y, Hirota K, Taniguchi-Yanai K, Nishijo N, Shigematsu Y, Horiguchi H, Kasuya Y, Sugiyama F, Yagami K i, Murakami K, Fukamizu A
J. Biol. Chem., 275, 5-8 (2000). 10617578

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ren1renin 1 structural1Ren1targeted mutation 1, Akiyoshi Fukamizu
  • familial juvenile hyperuricemic nephropathy type 2(MedGEN)

  • renal tubular dysgenesis of genetic origin(MedGEN)
  • lacZbeta-galactosidase (E. coli)1
  • familial juvenile hyperuricemic nephropathy type 2(MedGEN)

  • renal tubular dysgenesis of genetic origin(MedGEN)
  • neoneomycin resistance gene (E. coli)1
  • familial juvenile hyperuricemic nephropathy type 2(MedGEN)

  • renal tubular dysgenesis of genetic origin(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal blood homeostasis(MP:0009642)

  • abnormal innervation(MP:0002184)

  • abnormal juxtaglomerular apparatus morphology(MP:0002829)

  • abnormal juxtaglomerular cell morphology(MP:0011300)

  • abnormal macula densa morphology(MP:0011299)
  • more 11 phenotypes
  • cardiovascular system phenotype(MP:0005385)

  • decreased circulating renin level(MP:0003353)

  • decreased systemic arterial blood pressure(MP:0002843)

  • decreased systemic arterial diastolic blood pressure(MP:0006263)

  • decreased systemic arterial systolic blood pressure(MP:0006264)

  • hydronephrosis(MP:0000519)

  • kidney atrophy(MP:0004970)

  • polydipsia(MP:0001426)

  • polyuria(MP:0001762)

  • postnatal lethality, complete penetrance(MP:0011085)

  • vascular smooth muscle hyperplasia(MP:0005489)
  • Detailed phenotype data

    Ordering Information

    Donor DNAmouse renin 1 genomic DNA, E. coli neo, E. coli lacZ
    Research applicationCell Biology Research
    Hematological Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 275, 5-8 (2000).
    DepositorAkiyoshi Fukamizu (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data